Sierra College Department of Biological Sciences

Morphological Unknown Identification Assistance

Although determining the morphological characteristics of bacteria (cells and colonies) is a useful step in the identification process, students will find that gaining this information alone is insufficient for establishing the specific identity of microbial cultures. This Web-based laboratory activity is designed to provide additional information useful in the identification of unknown cultures. It will also introduce students to the National Center for Biotechnology Information (NCBI), a public resource containing a wealth of useful information.

To complete this assignment, click on the NCBI link provided and access the NCBI Home Page ( Click on "Nucleotide" under "Popular Resources", and then type (or copy and paste) the accession number listed for your unknown (to the right of your assigned number) into the "for" window. Hit return or click on the "go" button to access the required information.

Record the following information on your Morphological Unknown Report Form (Pg. 96):
1. The Database Accession number (near the top of the page, right hand side).
2. The technical name of your culture (Genus name and specific epithet).
3. The taxonomic lineage (listed just under the name), domain through species.
4. The name of the gene being investigated (recorded to the right of "Definition").
5. The name of the first author (recorded to the right of "Reference").
6. The country the author(s) is/are submitting from.

Morphological unknown web assisted identification:


Unknown # Accession # Unknown # Accession #
1 AJ865379 16 M85120
2 AY438619.1 17 GU142920.1
3 AM410859.1 18 GU339260
4 BK006794 19 AY167849
5 HQ825066.1 20 EF065663
6 GQ292756 21 FJ409226
7 AB018715 22 EF198139
8 AY876121 23 DQ001159
9 AM237362 24 E07193
10 JN585704.1 25 AY364316
11 AY299092.1 26 AM181587
12 DQ119337 27 GU383586.1
13 HM122242.1 28 NR_025475.1
14 AF214779 29 GQ411209.1
15 AJ581991 30 AB014173

NCBI Home Page

W3C Logo: Valid HTML 4.01 Transitional W3C Logo: Valid CSS