Sierra College Department of Biological Sciences

Morphological Unknown Identification Assistance

Although determining the morphological characteristics of bacteria (cells and colonies) is a useful step in the identification process, students will find that gaining this information alone is insufficient for establishing the specific identity of microbial cultures. This Web-based laboratory activity is designed to provide additional information useful in the identification of unknown cultures. It will also introduce students to the National Center for Biotechnology Information (NCBI), a public resource containing a wealth of useful information.

To complete this assignment, click on the NCBI link provided and access the NCBI Home Page ( Click on "Nucleotide" under "Popular Resources", and then type (or copy and paste) the accession number listed for your unknown into the "for" window. Hit return or click on the "go" button to access the required information.

Record the following information on your Morphological Unknown Report Form (Pg. 96):
1. The Database Accession number (near the top of the page, right hand side).
2. The technical name of your culture (Genus name and specific epithet).
3. The taxonomic lineage (listed just under the name), domain through species.
4. The name of the gene being investigated (recorded to the left of "Definition").
5. The name of the first author (recorded to the left of "Reference").
6. The country the author(s) is/are submitting from.

Morphological unknown web assisted identification:

Spring 2015

Unknown # Accession # Unknown # Accession #
1 AM237362 16 GU142920.1
2 AY438619.1 17 NR_025475.1
3 EF198139 18 JN585704.1
4 AY876121 19 E07193
5 M85120 20 AJ865379
6 FJ409226 21 DQ119337
7 AM181587 22 HM122242.1
8 AJ581991 23 AY299092.1
9 AB014173 24 EF065663
10 GQ292756 25 BK006794
11 AF214779 26 GU383586.1
12 DQ001159 27 AY364316
13 HQ825066.1 28 AY167849
14 AM410859.1 29 GU339260
15 AB018715 30 GQ411209.1

NCBI Home Page

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