Sierra College Department of Biological Sciences

Morphological Unknown Identification Assistance

Although determining the morphological characteristics of bacteria (cells and colonies) is a useful step in the identification process, students will find that gaining this information alone is insufficient for establishing the specific identity of microbial cultures. This Web-based laboratory activity is designed to provide additional information useful in the identification of unknown cultures. It will also introduce students to the National Center for Biotechnology Information (NCBI), a public resource containing a wealth of useful information.

To complete this assignment, click on the NCBI link provided and access the NCBI Home Page ( Click on "Nucleotide" under "Popular Resources", and then type (or copy and paste) the accession number listed for your unknown (to the right of your assigned number) into the "Search" window (above "Advanced"). Hit return or click on the "search" button to access the required information.

Record the following information on your Morphological Unknown Report Form (Pg. 90):
1. The Database Accession number (near the top of the page, right hand side, under the title).
2. The technical name of your culture (Genus name and specific epithet), in blue to the right of "organism".
3. The taxonomic lineage (listed just under the name), domain through species.
4. The name of the gene being investigated (recorded to the right of "Definition" and in the title), please write this out.
5. The name of the first author (recorded to the right of "Reference").
6. The country the author(s) is/are submitting from (listed to the right of "Journal" as part of an address).

Morphological unknown web assisted identification:

Fall 2016

Unknown # Accession # Unknown # Accession #
1 AY299092.1 16 AY876121
2 AB018715 17 FJ409226
3 M85120 18 DQ119337
4 AY167849 19 AF214779
5 GQ411209.1 20 BK006794
6 NR_025475.1 21 JN585704.1
7 AY364316 22 GU383586.1
8 HQ825066.1 23 EF065663
9 GU142920.1 24 AJ865379
10 AJ581991 25 HM122242.1
11 AM181587 26 GU339260
12 AM410859.1 27 DQ001159
13 E07193 28 EF198139
14 AY438619.1 29 AB014173
15 AM237362 30 GQ292756

NCBI Home Page

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