Sierra College Department of Biological Sciences

Morphological Unknown Identification Assistance

Although determining the morphological characteristics of bacteria (cells and colonies) is a useful step in the identification process, students will find that gaining this information alone is insufficient for establishing the specific identity of microbial cultures. This Web-based laboratory activity is designed to provide additional information useful in the identification of unknown cultures. It will also introduce students to the National Center for Biotechnology Information (NCBI), a public resource containing a wealth of useful information.

To complete this assignment, click on the NCBI link provided and access the NCBI Home Page ( Click on "Nucleotide" under "Popular Resources", and then type (or copy and paste) the accession number listed for your unknown into the "for" window. Hit return or click on the "go" button to access the required information.

Record the following information on your Morphological Unknown Report Form (Pg. 96):
1. The Database Accession number (near the top of the page, right hand side).
2. The technical name of your culture (Genus name and specific epithet).
3. The taxonomic lineage (listed just under the name), domain through species.
4. The name of the gene being investigated (recorded to the left of "Definition").
5. The name of the first author (recorded to the left of "Reference").
6. The country the author(s) is/are submitting from.

Morphological unknown web assisted identification:

Fall 2015

Unknown # Accession # Unknown # Accession #
1 AJ581991 16 BK006794
2 AF214779 17 AY167849
3 HM122242.1 18 AY364316
4 AB018715 19 HQ825066.1
5 DQ119337 20 GU142920.1
6 EF065663 21 AM181587
7 AJ865379 22 M85120
8 DQ001159 23 AY438619.1
9 NR_025475.1 24 GQ411209.1
10 FJ409226 25 AB014173
11 GU383586.1 26 AM410859.1
12 GU339260 27 JN585704.1
13 EF198139 28 AY299092.1
14 GQ292756 29 AM237362
15 E07193 30 AY876121

NCBI Home Page

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