Sierra College Department of Biological Sciences

Morphological Unknown Identification Assistance

Although determining the morphological characteristics of bacteria (cells and colonies) is a useful step in the identification process, students will find that gaining this information alone is insufficient for establishing the specific identity of microbial cultures. This Web-based laboratory activity is designed to provide additional information useful in the identification of unknown cultures. It will also introduce students to the National Center for Biotechnology Information (NCBI), a public resource containing a wealth of useful information.

To complete this assignment, click on the NCBI link provided and access the NCBI Home Page ( Click on "Nucleotide" under "Popular Resources", and then type (or copy and paste) the accession number listed for your unknown into the "for" window. Hit return or click on the "go" button to access the required information.

Record the following information on your Morphological Unknown Report Form (Pg. 96):
1. The Database Accession number (near the top of the page, right hand side).
2. The technical name of your culture (Genus name and specific epithet).
3. The taxonomic lineage (listed just under the name), domain through species.
4. The name of the gene being investigated (recorded to the left of "Definition").
5. The name of the first author (recorded to the left of "Reference").
6. The country the author(s) is/are submitting from.

Morphological unknown web assisted identification:

Fall 2014

Unknown # Accession # Unknown # Accession #
1 DQ001159 16 GQ292756
2 HQ825066.1 17 EF065663
3 AJ865379 18 AY876121
4 AF214779 19 AY167849
5 JN585704.1 20 HM122242.1
6 AM181587 21 E07193
7 GQ411209.1 22 BK006794
8 FJ409226 23 DQ119337
9 AM410859.1 24 AY364316
10 GU339260 25 EF198139
11 GU142920.1 26 NR_025475.1
12 GU383586.1 27 AB018715
13 AY299092.1 28 AJ581991
14 AB014173 29 AY438619.1
15 M85120 30 AM237362

NCBI Home Page

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