Sierra College Department of Biological Sciences

Microbiology Diagnostic Tests: Urea Hydrolysis or Urease Test

This medium is used to determine if or not bacteria are able to hydrolyze urea to form ammonia.


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1. Which of these tubes contains organisms that are capable of urea hydrolysis?

2. What is the name of the enzyme involved in this process?

3. What is the name of the pH indicator in this medium?

4. What change in pH is indicated by the color change in the positive tube, and what metabolic end product is responsible?

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