Microbiology Diagnostic Tests: Oxidation / Fermentation of O/F Test
This test is used to determine if bacteria are using a fermentative or a respiratory (oxidative) type of metabolism. It also provides information about the gas requirements of the organisms present.
Tube Sets:
------------------A ------------------------------------------B--------------
1. Which tube set contains organisms that are fermentative?
2. What are the fermentation products present, and how are these products indicated, i.e., what evidence of fermentation is visible?
3. What is apparent about the gas requirements of the fermentative organisms?
4. What is the name of the pH indicator in this medium?
5. Which tube set contains organisms that are using a respiratory (oxidative) type of metabolism?
6. What can be assumed about the gas requirements of the respiratory organisms?
7. What microbial activity is responsible for the color change observed on the surface of the unsealed tube in tube set B?
Copyright 2002 Sierra College Biological Sciences Department
Last updated: 02/11/02.
