Sierra College Department of Biological Sciences

Microbiology Diagnostic Tests: Citrate Utilization

This test is used to determine if or not bacteria are capable of utilizing citrate as their sole source of carbon. The Simmons Citrate medium contains sodium citrate as the only carbon source.


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1. Which of these tubes contains organisms that are capable of taking in and utilizing citrate?

2. What is the name of the pH indicator in this medium?

3. What change in pH is indicated by the color change in the positive tube, and what metabolic end product is responsible?

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