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					Nucleotide Sequencing Results - CULTURE # 9 
						 
					 
					  Culture #9 - Set A 
					
						
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								 gaattccgcctttctttgctttttgattattatacaaaaaaaaagacggtgttcaaaagaaaaactcatgaaaaaacgcctcagctttcactgaa 
									acgcctttttttatttatctttaataaaaatagcaatgatgtcaaaaaatagtcgattttactaagtgatgaattatgggtggtacaaatactgctgg 
									aaatagttgttaacttcaagggcatcttctttattttttgaaagaattaagatggtatcatccccagcgatgcagcccaagatatgagggttgtgg 
									atacgtatgcgatcaattaaaaccccaatggcttgcccgttgccaggtaagacggttaaaagatttgtgaactctatttgtgttaaagaaacacca 
									gtttcaataataatgtcaacaagacgttcttcatctgttttatattttggacgaagggcagaattattcaagatacgataataggcttctccttgacca 
									ttgtctactttgtttaaattcagctcacgaatgtcaccagaaatagttgcttgtgtggtgtgaatattttctttttccaataatgctagtaattcactttgtg 
									tttggatttcattttctgtgaccaatcgcttaatcatcgtttggcgttttaatcggttcattactttcctccagttcagttaatcatgcaaaaaaacatcatt 
									gctgttatttaaaacggtgccagtaattccctaaaatcactgtacaacgttttactaaattgtgcaagtctcacttttttaaatgcgaaatatccgaca 
									aaatcaatgagaaccttttagctaaagcaccctttttctattctaattagagagattctgctagtattgctttgtaaatgtctggaggaagggtgtta 
									tttgtgaataagc 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	What is carbamate kinase and what does it do? 
						2.	The gene for this enzyme in this bacteria is identical in nucleotide sequence to a gene from what other type of bacteria? 
						3.	What other enzyme has been found to have a similar structure and perhaps share a common catalytic mechanism with this one? 
						 
						 
						 
					 
					
					  Culture #9 - Set B 
					
						
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								 gaggccgatagtctggaaaaactcgaagccctgctaccctggaacgtcccgctggagcccattgcaaaaaaagtggcgcaatacaacgaa 
									ggcaagtaggtcgatttagcggcgcttacgggagaacagcttgccgggcttgcatcggccatgcgctaatcctgtggcataaccataagggg 
									ataaacaatgtggcagtggcttggaaaagtgcggtggttgctggtggtgatagcgctgctgggtggcaaggtcatggcggcgtctgaactgg 
									atagcgcgctggccagactcgagcagcagcatcatgggcgtctggggctggcctatatcgatagtggcagcggcgagagttacagctatcgc 
									ggtgaggagcgttttgccttttgcagcaccttcaaggcggtgctggcgggggcggtgctgcagcgaagcgtcagtcagcccggtttgctcgata 
									agcgggtgcattatgcggccacggatctgctcgcctatgcccccatcaccaagacgcatctggataaagggatgcgcatcggtgaactggccg 
									cagcggcggtgcaatatagtgacaacacggcggccaacctgctgctgcaagaaattggcggcgtccaggcgctgaaccggtttgtgcaggg 
									gttgggagatccggcattccgtctggataggatagagcctcaccttaatagtgccgagccaggagatgtgcgcgataccaccacgccgctggc 
									catggcccacaccctgcaggccatgactctgggcaaggggtta 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	Where were ESBLs first characterized for members of the family Enterobacteriaceae? 
						2.	What are ESBLs and what do these enzymes do? 
					 
					 
					 
					 
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