Bio. Sci. 4

Nucleotide Sequencing Results - CULTURE # 7

Culture #7 - Set A

acaatatcagatatgaaacataaagtgttaggagcacaatcagcttcatcaggttatgactccttgttaagaactcctaaactgctgaaagattttatt
aaaaataaagacgctaatcaatatgaaacctttacacaagcttttattgatttaaaatcagatcgtatcgatggaatattgattgacaaagtatatgcc
aattactatttagcaaaagaagggcaattagagaattatcggatgatcccaacgacctttgaaaatgaagcattttcggttggacttagaaaagaag
acaaaacgttgcaagcaaaaattaatcgtgctttcagggtgctttatcaaaatggcaaatttcaagctatttctgagaaatggtttggagatgatgttgc
cactgccaatattaaatcttaaaaaggacacttgtcctttttttgtttaacaggtgagatctagcagacaaagtcagctagacgatttaataaaaatctta
gtttaaggttttttattaaactgtattttttctgaaaattatag

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence and answer the following questions.
1. What is the CAMP reaction?
2. What is the name of the bacteria that produce sphingomyalinase C (beta toxin)?

Culture #7 - Set B

ccaggatcctggcgagacactataagcaggcgctggaaggacaaatatcgaattgatgatggaattccatcatcaataaattgcttgtcaaaata
gcgggagtcgcagataatccgctgcgacttatcactctcaaggaatcagaaatgataaaaagttcgtggcgtaaaattgcaatgctagccgc
cgttccgctgctgctggcgagcggcgcactgtgggccagtaccgatgctatccatcagaagctgacagatctcgagaagcgttcaggcggc
aggttgggcgtggcgctaatcaacacggcagataattctcaaatcttatatcgcggcgacgagcgttttgccatgtgcagcaccagtaaagtg
atggccgccgccgcggtattaaaacagagcgaaagcaataaagaggtggtaaataaaaggctggagattaacgcagccgatttggtggtct
ggagtccgattaccgaaaaacatctccagagcggaatgacgctggctgagctaagcgcggcgacgctgcaatatagcgacaatacggcgat
gaatctgatcatcggctaccttggcgggccggaaaaagtcaccgccttcgcccgcagtatcggcgatgccacctttcgtctcgatcgtacggagc
ccacgctgaataccgccatcccgggcgatgagcgtgataccagcacgccgctggcgatggctgaaagcctac

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. Since the early 1980s these bacteria have been recognized as what?
2. In wild type strains of these bacteria, chromosomal b-lactamases are constitutively produced at low levels and affect the organisms susceptibility to ___________________ while overproduction confers resistance to what?