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					Nucleotide Sequencing Results - CULTURE # 6 
						 
					 
					  Culture #6 - Set A 
					
						
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								 aagcttaattatcaataatgaactaggtaacatactgtccataaaagtacacttcaagaaaaatagtgcacttagacgtaagaagtttacttgga 
									gagatgacgctaatagtaagaagacgagtggatcattgggggatctgggtattcaccttattgacatggtatggtatttgttcgagagtgatttcat 
									cacagaatcagtaagggcaaagatgaacacaaatgtaaaaacaaaagaggataaacaggtacttgtagatgactatgcagaaatttatggc 
									cagctgaagaacaaggtatttgtaaatatcatcacatcaaagtgttctgtacctgaagactgtggttttagcattgaggtagttggacacaaaaaa 
									gagtttaaataccacacaggtaatcctcacgtttacaagctcatagatggcttgaacgtggtagactgcccagtaccgcaaagcctattaaacgat 
									ccgccaaacgagttttatggatgggctgattcttttagaagcgagttaatcaattggattgcatcaactcagaatgattgggttgagatcccttctttta 
									gtgatggttttagatctcaggaagtattagaaatgttctttgagaaagacagcaactctcaacccatgtctgtttcagcagtcaactagtatttcaaag 
									agagaagttactaaaaaagcaggaatttactttcctgctttttcatataggggtgtaatgaatgttaagagggacatatttatttggatatgctttcttttt 
									tacagtaggtattatccatatatcaacagggagtttgacaccatttttattagaggcttttaac 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	Catabolite repression in these organisms involves a catabolite control protein A (CcpA) which interacts with __________________________________________________________ to effect catabolite repression by a negative control mechanism. 
						2.	What is the product of the newly identified gene glcP, and what is the function of this protein? 
						3.	The mutant bacteria presented in this paper did not display glucose mediated catabolite repression, but they exhibited strong repression in response to what? (See discussion section.) 
						 
						 
						 
					 
					
					  Culture #6 - Set B 
					
						
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								 atgatgaaaaaatcgatatgctgcgcactgctgctgacagcctctttctccacgtttgctgccgcaaaaacagaacaacaaattgccgatatcgttaa 
									ccgcaccatcacaccactgatgcaggagcaggctattccgggtatggccgtggcgattatctacgaggggaaaccttattactttacctggggtaaag 
									ccgatatcgccaataaccacccagtcacgcagcaaacgctgtttgagctagggtcggtcagtaagacgtttaacggcgtgttgggcggcgacgctatc 
									gcccgcggcgaaattaagctcagcgatccggtcacgaaatactggccagaactgacaggcaaacagtggcggggtatcagcctgctgcacttagcc 
									acctatacagcgggtggcctgccgctgcagatccccgatgacgttacggataaagccgaattactgcgcttttatcaaaactggcaaccacaatggact 
									ccgggcgctaagcgtctttacgctaactccagcattggtctgtttggtgcgctggtggtaaaaccttcaggtatgagctacgaagaggcaatgaccagac 
									gcgtcctgcaaccattaaaactggcgcatacctggattacggttccgcaaagcgaacaaaaaaattatgcctggggctatcgcgaagggaagcctgta 
									cacgtttctccgggacaacttgacgccgaagcctatggcgtgaaatccagcgttatcgatatggcccgctgggttcaggccaacatggacgcc 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1. Which antibiotics are the most widely used currently (accounting for 50% of the antibiotics used globally)? 
						2. The Amp-C b-lactamases are primarily _________________ that mediate resistance to what? 
					 
					 
					
					 
					 
					 
					 
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