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					Nucleotide Sequencing Results - CULTURE # 5 
						 
					 
					  Culture #5 - Set A 
					
						
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								 ctatttggggtgagtagataattgagccgcttttcaaactttttaatggtgaaaaaataatccatctcactcttgatgaagtaagacggattatt 
									gttgtttaaattagtactgtcttttaaagtgctttaaaaagagattgagcaggtatagtatttccttagtatttgttattctatgaaaatctctagttta 
									aatcaatttatgtatgttattttcctattcaggtttatggttcaaatcattatctctaaaaaactagacattggagtaaaaggtatcatttatctgaa 
									tattgtaattatgatctcaaaaccgtagaaaatagtgaagatatgatttacctattttgtactgtttcttaatgttaattttgttcctagtttagtaagtg 
									ttggtatttttcgaggtgctaataattgtttattgagaatatccatagctgttcgtcccatttcttctgtatagacagtgacactagaaagtggaggat 
									acacttgtttagctagagttgtatcgttaaaagagataatttgaatgtcgtcagggaccttgataccattttcctgaagtgctctgagtgcaccaa 
									tagctagactatcactagccgcaaagtaagcatcaggtaaagtagctccactcttaatcttagaatcaagaagttcatagccagattggacag 
									tgaagtcaccagtcagaataaaaagagggtcatagattcccttttccatacagtagttccgataagaacgtaaacgaggatcagagatgattt 
									cagttgcatctgttgttttttcttgcccaatgagtaggcctatattattacaaccttgttctttaagataacagagtgccgattgtacggagttttcaaa 
									atcagtagtaacacatggatgtcct 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	When was the lactose operon of E. coli discovered? 
						2.	Where is the b-galactosidase gene of this bacteria located relative to the permease gene? 
						3.	Cultures of this bacteria do not usually ferment galactose.  Is this because they lack genes coding for enzymes that would allow galactose utilization to occur, or is it because these genes are not being induced?  
						 
						 
					 
					
					  Culture #5 - Set B 
					
						
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								 aagcttttgtgagccgtcgaagtgctcatgacaaggcgaagccgcagtcggggtgtatctccgcgttagcgagccgtaagggctgcttac 
									gggcgtgttattcaataccttcagtgatatatcctacagcaactcaggactctagttacaacataatccatcacaggcaacgcgcctgcttttg 
									gctcccctaatacctgtgtcttcagccaagatgatgacagttatcggggggcattcaggttccgttaaatcccgttacctgttcctgaatgtttg 
									aacggacatgtcctgtctctgtttgttttcggaagcccgcatttcttccgaaaaaccatttttttgctacccgcctgactcaccacaagcaataag 
									tctggaaacccgaggggcacagcatgctgcgcccctacgtattatcttctgccgcttctttcagtaaccattcgctaaatatttccatcgccggg 
									gtcattgctttcgattttaaatgggttaaccagtaactgcccatattgatttcggtagcaaatgggcgcaccagctgcgcggtggttagctcccg 
									gacaaacattttcgctggggctaatgccacaccgccggaatggatagcgctctcgatcatcagacgggatgagtcaaagatggcgccattc 
									actctcacagcctgcaagccagcggcagcaaaccagttttcccactcttcagcacggtaagaac 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	How have Gram-negative bacteria responded to the clinical use of extended-spectrum cephalosporins? 
						2.	Overproduction or constitutive production of inducible chromosomal b-lactamase causes what in these bacteria? 
						3.	These bacteria are known to gain resistance to b-lactams easily because of mutations in what? 
						 
						 
					 
					
					 
					 
					 
					 
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