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					Nucleotide Sequencing Results - CULTURE # 4 
						 
					 
					  Culture #4 - Set A 
					
						
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								 tagctttgtacgatagtacttaattaatgaaccaatcttcattttcataacctcccgtatcgaatgactgtattttacatttattataatcataattttc 
									ccaataatcaaaaaacctaaattatggtagattcaaattgtagtactataacttaatttgaaaaggagttggaaagaatgaaaaaaatgaa 
									aagtacgttgctaagtcttttgattgcaggatcagtaataaacccggtgtacgcaacagcgatggatagcgaagatagcttaggaaacgac 
									ggtggtcaggagaaattccgtgtttatgttgaggcaaacaaaaaaacagaaaaatcaacattaaaaactcaatattctgctcgttgggaatt 
									aacagagaacggattttcaactgatatgaatgaaaaacaattcgaagcattacagaagaataaaaatttcacagttacaaaagtgcctatcg 
									ttacattagatacgataggtttggaagacgatagtatggtagaaaatgaaactctggatttggtaacttctttaagagcttctcaacaaatacct 
									tggggcattaaagcaatttataacaatgatactttaacatctacaactggaggaagtggcattaatattgccgtactcgatacgggtgtaaata 
									cttctcatcctgaccttgtcaataacgtagagcaatgtaaagactttacaggtgcgacaacacccattaataacagttgtactgatagaaacgg 
									acatggtacacatgttgctggtacggccttagctgatggtggaagcgatcaagcaggcatttacggtgtagctccagatgctgatctttgg 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	These bacteria have been used along with B. thuringiensis to control what? 
						2.	Why is it desireable to combine multiple toxin genes in a single population of bacteria? 
						3.	Why are these organisms considered to be the best host for combining multiple toxin genes? 
					 
					 
						 
					 
					
					  Culture #4 - Set B 
					
						
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								 ttgtgaatcagcaaaacgccgggttattcttatttgtcgcttctttactcgcctttatcggccctcactcaaggatgtattgtggttatgcgttattt 
									tcgcctgtgtattatctccctgttagccaccctgccgctggcggtacacgccagcccgcagccgcttgagcaaattaaactaagcgaaagc 
									cagctgtcgggcagcgtaggcatgatagaaatggatctggccagcggccgcacgctgaccgcctggcgcgccgatgaacgctttcccat 
									gatgagcacctttaaagtagtgctctgcggcgcagtgctggcgcgggtggatgccggtgacgaacagctggagcgaaagatccactatc 
									gccagcaggatctggtggactactcgccggtcagcgaaaaacaccttgccgacggcatgacggtcggcgaactctgtgccgccgccatta 
									ccatgagcgataacagcgccgccaatctgctgctggccaccgtcggcggccccgcaggattgactgcctttttgcgccagatcggcgacaa 
									cgtcacccgccttgaccgctgggaaacggaactgaatgaggcgcttcccggcgacgcccgcgacaccactaccccggccagcatggccg 
									cgaccctgcgcaagctgctgaccagccagcgtctgagcgcccgttcgcaacggcagctgctgcagtggatggtggacgatcgggtcgccg 
									gaccgttgatccgctccgtgctgccggcgggctggtttatcgccgataagaccggagctgccaaacggggtgcgcgcgggattgtcgccctg 
									cttggcccgaataacaaagcagagcggattgtggtgatttatctgcgggatacgccggcgagcatggccgagcgaaatcag 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	What are ESBLs and what is their significance? 
						2.	The bacteria that carry genes coding for ESBLs are frequently associated with what types of infections?  
					 
					
					 
					 
					 
					 
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