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					Nucleotide Sequencing Results - CULTURE # 3 
						 
					 
					  Culture #3 - Set A 
					
						
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								 tggagatacggctctttctgcaaatgaagcgagaatgaaagagaccttgcaaaaggctggattatttgcaaaatctatgaatgcctattcttatatgt 
									taattaagaatcctgatgtaaattttgaaggaattactattaatggatatgtagatttacctggtagaatcgtacaagatcaaaagaatgcaagagca 
									catgctgttacttgggacacgaaagtaaaaaaacagcttttagatacattgactggtattgttgaatatgataccacgttcgacaattattatgaaac 
									attggttgaggcgattaatacaggggatggagaaactttaaaagaaggaattacagatttacgaggtgaaattcaacaaaaccaaaagtacgca 
									caacaattaatagaagaattaactaaattaagagactctattggacaagatgttagagcatttggaggcaataaagatcttttgcaatcgattctaaa 
									aaaccaaggtgcagatgttgatgcagatcaaaagcgtctagaagaagtattaggatcagtaaactattataaac 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	What four species belong within this group of bacteria? 
						2.	Which newly described species is psychroduric? 
						3.	What symptoms are associated with the emetic toxin and enterotoxins produced by these bacteria? 
						 
						 
					 
					
					  Culture #3 - Set B 
					
						
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								 atggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcg 
									tctgtcttctggcttgcgtattaacagcgcgaaggatgacgccgcaggtcaggcgattgctaaccgttttacttctaacattaaaggcctgactc 
									aggcggcccgtaacgccaacgacggtatttctgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtattcg 
									tgaactgacggttcaggccactacagggactaactccgattctgacctggactccatccaggacgaaatcaaatctcgtcttgatgaaattga 
									ccgcgtatccggccagacccagttcaacggcgtgaacgtgctggcgaaagacggttcaatgaaaattcaggttggtgcgaatgacggcgaa 
									accatcacgatcgacctgaaaaaaatcgattctgatactctgggtctgaatggctttaacgtaaatggtaaaggtactattaccaacaaagct 
									gcaacggtaagtgatttaacttctgctggcgcgaagttaaacaccacgacaggtctttatgatctgaaaaccgaaaataccttgttaactaccg 
									atgctgcattcgataaattagggaatggcgataaagtcacagttggcggcgtagattatacttacaacgctaaatctggtgattttactaccacta 
									aatctactgctggtacgggtgtagacgccgcggcgcaggctgctgattcagcttcaaaacgtgatgcgttagctgccacccttcatgctgatgtg 
									ggtaaatctgttaatggttcttacaccacaaaagatggtactgtttctttcgaaacggat 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	What do genes located at the fliC locus code for in the subject bacteria? 
						2.	Which antigens are most frequently used to identify different strains of these bacteria? 
						3.	What type of disease is caused by these bacteria in the strain O157:H7 and how were these organisms transmitted to 477 U.S. residents in 1993?  
						 
						 
					 
					
					 
					 
					 
					 
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