| 
					  
					 
					Nucleotide Sequencing Results - CULTURE # 26 
						 
					 
					  Culture #26 - Set A 
					
						
							| 
								 tagctttgtacgatagtacttaattaatgaaccaatcttcattttcataacctcccgtatcgaatgactgtattttacatttattataatcata 
									attttcccaataatcaaaaaacctaaattatggtagattcaaattgtagtactataacttaatttgaaaaggagttggaaagaatgaaa 
									aaaatgaaaagtacgttgctaagtcttttgattgcaggatcagtaataaacccggtgtacgcaacagcgatggatagcgaagatagc 
									ttaggaaacgacggtggtcaggagaaattccgtgtttatgttgaggcaaacaaaaaaacagaaaaatcaacattaaaaactcaatat 
									tctgctcgttgggaattaacagagaacggattttcaactgatatgaatgaaaaacaattcgaagcattacagaagaataaaaatttca 
									cagttacaaaagtgcctatcgttacattagatacgataggtttggaagacgatagtatggtagaaaatgaaactctggatttggtaactt 
									ctttaagagcttctcaacaaataccttggggcattaaagcaatttataacaatgatactttaacatctacaactggaggaagtggcattaa 
									tattgccgtactcgatacgggtgtaaatacttctcatcctgaccttgtcaataacgtagagcaatgtaaagactttacaggtgcgacaaca 
									cccattaataacagttgtactgatagaaacggacatggtacacatgttgctggtacggccttagctgatggtggaagcgatcaagcagg 
									catttacggtgtagctccagatgctgatctttgg 
							 | 
						 
					 
					
					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	These bacteria have been used along with B. thuringiensis to control what? 
						2.	Why is it desireable to combine multiple toxin genes in a single population of bacteria? 
						3.	Why are these organisms considered to be the best host for combining multiple toxin genes? 
						 
					 
					 
						 
					 
					
					  Culture #26 - Set B 
					
						
							| 
								 ccaggatcctggcgagacactataagcaggcgctggaaggacaaatatcgaattgatgatggaattccatcatcaataaattgct 
									tgtcaaaatagcgggagtcgcagataatccgctgcgacttatcactctcaaggaatcagaaatgataaaaagttcgtggcgtaaa 
									attgcaatgctagccgccgttccgctgctgctggcgagcggcgcactgtgggccagtaccgatgctatccatcagaagctgacag 
									atctcgagaagcgttcaggcggcaggttgggcgtggcgctaatcaacacggcagataattctcaaatcttatatcgcggcgacga 
									gcgttttgccatgtgcagcaccagtaaagtgatggccgccgccgcggtattaaaacagagcgaaagcaataaagaggtggtaaa 
									taaaaggctggagattaacgcagccgatttggtggtctggagtccgattaccgaaaaacatctccagagcggaatgacgctggct 
									gagctaagcgcggcgacgctgcaatatagcgacaatacggcgatgaatctgatcatcggctaccttggcgggccggaaaaagtc 
									accgccttcgcccgcagtatcggcgatgccacctttcgtctcgatcgtacggagcccacgctgaataccgccatcccgggcgatgag 
									cgtgataccagcacgccgctggcgatggctgaaagcctac 
							 | 
						 
					 
					
					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	Since the early 1980s these bacteria have been recognized as what? 
						2.	In wild type strains of these bacteria, chromosomal b-lactamases are constitutively produced at low levels and affect the organisms susceptibility to ___________________ while overproduction confers resistance to what? 
						 
					 
					 
					 
					 
					 
					 
					 
				 |