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					Nucleotide Sequencing Results - CULTURE # 22 
						 
					 
					  Culture #22 - Set A 
					
						
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								 aagcttaattatcaataatgaactaggtaacatactgtccataaaagtacacttcaagaaaaatagtgcacttagacgtaagaagttta 
									cttggagagatgacgctaatagtaagaagacgagtggatcattgggggatctgggtattcaccttattgacatggtatggtatttgttcga 
									gagtgatttcatcacagaatcagtaagggcaaagatgaacacaaatgtaaaaacaaaagaggataaacaggtacttgtagatgact 
									atgcagaaatttatggccagctgaagaacaaggtatttgtaaatatcatcacatcaaagtgttctgtacctgaagactgtggttttagcatt 
									gaggtagttggacacaaaaaagagtttaaataccacacaggtaatcctcacgtttacaagctcatagatggcttgaacgtggtagactg 
									cccagtaccgcaaagcctattaaacgatccgccaaacgagttttatggatgggctgattcttttagaagcgagttaatcaattggattgca 
									tcaactcagaatgattgggttgagatcccttcttttagtgatggttttagatctcaggaagtattagaaatgttctttgagaaagacagcaac 
									tctcaacccatgtctgtttcagcagtcaactagtatttcaaagagagaagttactaaaaaagcaggaatttactttcctgctttttcatatagg 
									ggtgtaatgaatgttaagagggacatatttatttggatatgctttcttttttacagtaggtattatccatatatcaacagggagtttgacacca 
									tttttattagaggcttttaac 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	Catabolite repression in these organisms involves a catabolite control protein A (CcpA) which interacts with __________________________________________________________ to effect catabolite repression by a negative control mechanism. 
						2.	What is the product of the newly identified gene glcP, and what is the function of this protein? 
						3.	The mutant bacteria presented in this paper did not display glucose mediated catabolite repression, but they exhibited strong repression in response to what? (See discussion section.) 
						 
						 
						 
						 
					 
					
					  Culture #22 - Set B 
					
						
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								 gcggagtacatcgcgttctgcacgcgctccggctgcgtctgcagcgagaccaattggctgttgtaggtcgcaagatcgttctgcgcgctttgcag 
									atcgtcgagcgcctgagacaccctggattcaagctgtcgcaacgataacgtgctcagggttttacgggtgacctcatcatcaggaacgtcgctca 
									gcgcattcaggctgtctaccgcctgacgcattttcgccggcgcctgttcaacctgctgacgcagttgcacagtttcagactttatacgctcaatctt 
									atcgagcgtttctaaagtctgcgtcaggtcctgctgaactaatttttcctgcggcgtgagttctttttgcttatttagcgtagtgagctggctttgaatgt 
									cagaacggtcaggtaaatctgcagcctgaacctggctgaagctcagagtgccaactataaggaccagtgcaaagacaatggtggaaatagc 
									atgaggccaacgatttgtatgctgcatagtattaacatgaatggttgcaaatgccggagaataccgggggtcgtcgcagcgcgcagaatagca 
									cttctccctgcgcgcgaatagcggcagagataagtaaaatttacctttatttacgcctgcggattcaggccatgacctcagacgaacttcgtagcg 
									acgggcaggtctgatacatctcaatcagcgcatcgacataagcacgggcttc 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	For the last decade, these commensal Gram-negative bacteria have been rapidly emerging as what? 
						2.	The pumps used by these bacteria to remove disinfectants and antibiotics from their cytoplasm (cytosol) use what as a source of energy? 
						 
					 
					 
					 
					
					 
					 
					 
					 
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