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					Nucleotide Sequencing Results - CULTURE # 21 
						 
					 
					  Culture #21 - Set A 
					
						
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								 gaattccgcctttctttgctttttgattattatacaaaaaaaaagacggtgttcaaaagaaaaactcatgaaaaaacgcctcagctttcactg 
									aaacgcctttttttatttatctttaataaaaatagcaatgatgtcaaaaaatagtcgattttactaagtgatgaattatgggtggtacaaatact 
									gctggaaatagttgttaacttcaagggcatcttctttattttttgaaagaattaagatggtatcatccccagcgatgcagcccaagatatgagg 
									gttgtggatacgtatgcgatcaattaaaaccccaatggcttgcccgttgccaggtaagacggttaaaagatttgtgaactctatttgtgttaaa 
									gaaacaccagtttcaataataatgtcaacaagacgttcttcatctgttttatattttggacgaagggcagaattattcaagatacgataatag 
									gcttctccttgaccattgtctactttgtttaaattcagctcacgaatgtcaccagaaatagttgcttgtgtggtgtgaatattttctttttccaataat 
									gctagtaattcactttgtgtttggatttcattttctgtgaccaatcgcttaatcatcgtttggcgttttaatcggttcattactttcctccagttcagtta 
									atcatgcaaaaaaacatcattgctgttatttaaaacggtgccagtaattccctaaaatcactgtacaacgttttactaaattgtgcaagtctca 
									cttttttaaatgcgaaatatccgacaaaatcaatgagaaccttttagctaaagcaccctttttctattctaattagagagattctgctagtattgc 
									tttgtaaatgtctggaggaagggtgttatttgtgaataagc 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	What is carbamate kinase and what does it do? 
						2.	The gene for this enzyme in this bacteria is identical in nucleotide sequence to a gene from what other type of bacteria? 
						3.	What other enzyme has been found to have a similar structure and perhaps share a common catalytic mechanism with this one? 
						 
						 
						 
						 
					 
					
					  Culture #21 - Set B 
					
						
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								 tctagatacaccatatggcctagcaacatcagatacaggaatagaaccatctttgagtatgccctaatctccctgatatcctttttctg 
									taactttggcttccgcccacccaagcgaccgcgagctcttgcggcttccaatcccgccttggtacgttcgctaatcaaatttcgctcaa 
									attcagccaaagcagcaaacacatgaaaaaccagctttccagaagcactgttcgtttcaatctgttcttgtaggctcagaaatccaa 
									tccgtcgctcatcaaccagatgggtaacaacgctcaccaagtctgccaaactacggcctaacctatccagcctccaaacgaccaa 
									cgtatccccaggtctaagagcctttaagcagttatcaagttcgggtctagcggcattcttcccactggccttgtcctcgtaaatgatctc 
									gcaaccagcagcagccaacgcatctctctgcaaatgcaggttctgatcgtcggttgaaaccctagcgtagccaattttttgattcatg 
									tacgtttttttaacatagaattttatacaagtattttaacatgtatatagattaaattccaatcaacttgtgtcgttttcagaagacggctgc 
									actgaacgtcagaagccgactgcactatagcagcggag 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions.   
						1.	What antibiotics were these organisms resistant to?   
						2.	What mechanisms (structures) were proposed for the transfer of resistance genes?  
						 
						 
					 
					
					 
					 
					 
					 
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