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					Nucleotide Sequencing Results - CULTURE # 20 
						 
					 
					  Culture #20 - Set A 
					
						
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								 aaattcttctttttatgcaaccgcttgctctatactatttttgtagacgtttacaaaatgttgtcgaattagactatgcttttggggaggatgatcg 
									gtttgaccttgtatcgaagtctatggaaaaaagggtgtatgcttctactaagccttgtgctgtctctgaccgccttcattggctcaccctctaat 
									acggcgagcgccgcggttgcagatgattttcaggcttccgttatgggaccactggcgaaaatcaacgactggggttcctttaaaaagcaa 
									ctgcaaacgttgaaaagcaatggcgtgtatgccatcactaccgacgtctggtggggctatgtggagagcgcaggagataaccagttcgat 
									tggagctactacaaaacctatgcagatgccgtgaaagaagcgggactgaagtgggttcctattatctctacgcataagtgtggaggaaat 
									gtgggagatgattgcaacatcccactgccaagctggctgtcaagcaagggtagtgcagatgaaatgcagttcaaggacgaaagcggct 
									atgccaataacgaagccctttccccattgtggagcgggactggcaaacaatatgacgagttgtatgcctcattcgctcaaaactttgccggg 
									tataaaagtatcattcccaaaatttatttaagcggcggtccttccggggaattgcgctatccgtcctactacccggcagcgggctggagttacc 
									cgggacgaggcaagttccaagcctatactgaaacagccaaaaagtcattccgcacagctatgaatgacaagtacggatcattggacaa 
									gattaatgctgcttgg 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	The gene coding for beta amylase was cloned in what type of bacteria? 
						2.	Does the sequence shown contain a translational stop codon?  If not; why not? 
						3.	What type of sequence occurs immediately upstream of the signal sequence indicated by italics (coding for 38 amino acids)? 
						 
						 
						 
					 
					
					  Culture #20 - Set B 
					
						
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								 gaggccgatagtctggaaaaactcgaagccctgctaccctggaacgtcccgctggagcccattgcaaaaaaagtggcgcaat 
									acaacgaaggcaagtaggtcgatttagcggcgcttacgggagaacagcttgccgggcttgcatcggccatgcgctaatcctgtg 
									gcataaccataaggggataaacaatgtggcagtggcttggaaaagtgcggtggttgctggtggtgatagcgctgctgggtggca 
									aggtcatggcggcgtctgaactggatagcgcgctggccagactcgagcagcagcatcatgggcgtctggggctggcctatatcg 
									atagtggcagcggcgagagttacagctatcgcggtgaggagcgttttgccttttgcagcaccttcaaggcggtgctggcgggggc 
									ggtgctgcagcgaagcgtcagtcagcccggtttgctcgataagcgggtgcattatgcggccacggatctgctcgcctatgccccca 
									tcaccaagacgcatctggataaagggatgcgcatcggtgaactggccgcagcggcggtgcaatatagtgacaacacggcggc 
									caacctgctgctgcaagaaattggcggcgtccaggcgctgaaccggtttgtgcaggggttgggagatccggcattccgtctggat 
									aggatagagcctcaccttaatagtgccgagccaggagatgtgcgcgataccaccacgccgctggccatggcccacaccctgcag 
									gccatgactctgggcaaggggtta 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	Where were ESBLs first characterized for members of the family Enterobacteriaceae? 
						2.	What are ESBLs and what do these enzymes do? 
						 
						 
						 
					 
					
					 
					 
					 
					 
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