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					Nucleotide Sequencing Results - CULTURE # 2 
						 
					 
					  Culture #2 - Set A 
					
						
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								 atgaaaaaaacagcatttacattacttttattcattgccctaacgttgacaacaagtccacttgtaaatggtagcgagaaaagcgaagaaataaatg 
									aaaaagatttgcgaaaaaagtctgaattgcagggaacagctttaggcaatcttaaacaaatctattattacaatgaaaaagctaaaactgaaaa 
									taaagagagtcacgatcaatttttacagcatactatattgtttaaaggcttttttacagatcattcgtggtataacgatttattagtagattttgattcaaa 
									ggatattgttgataaatataaagggaaaaaagtagacttgtatggtgcttattatggttatcaatgtgcgggtggtacaccaaacaaaacagcttgtat 
									gtatggtggtgtaacgttacatgataataatcgattgaccgaagagaaaaaagtgccgatcaatttatggctagacggtaaacaaaatacagtacct 
									ttggaaacggttaaaacgaataagaaaaatgtaactgttcaggagttggatcttcaagcaagacgttatttacaggaaaaatataatttatataactctg 
									atgtttttgatgggaaggttcagaggggattaatcgtgtttcatacttctacagaaccttcggttaattacgatttatttggtgctcaaggacagtattcaaat 
									acactattaagaatatatagagataataaaacgattaactctgaaaacatgcatattgatatatatttatatacaagttaa 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						Note - Students must access the full-text article from Lancet to answer the questions. 
						1.	Describe the BLA Operon (name the genes present, what they code for, and what functions are associated with the proteins coded for). 
						2.	How do leukocidins kill phagocytic WBCs? 
						3.	What are superantigens? 
						 
						 
						 
					 
					
					  Culture #2 - Set B 
					
						
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								 gcgaaaaattaattgttatctctttttttatctacttataaattgacctttttcctaaaaagcaaagcccaaaattggcgatgatatatggcg 
									ttattatcgccatcgacttgaataattatttattaaaataataagtagagctagtgtatttattatcagggaggttcagatatagctaattatggat 
									aataagcgaacacagcgggatattatatttagcataatatggattatttggtcatgtgcattaatggctttttggcgatatcaaatagctggtga 
									gcttttgatcacccttatcctaatttttagcattattatttactctataactttgaggttaaaaaaaacggcaatgtttagtcgaaaaacagaaac 
									accaaaagccgcggagcctattgctccagttatggcagaagagaagaagcctatgccggagcaaaaattgtataccattattgctaaagg 
									cactgtatttcaaggtgatattaacgttgatggtgatattcaaatttggggtaaaatttcagggaatatcaatgtaaaagatggcgtgatccgt 
									gttatgcatgcaggccaagttgaaggggaattgacggcgccagacatcattattgatggttttgttaaaggtatttgtgccgcaaacaatcttg 
									atattctagagcatggtgaactacgtggcactagtcgttgtggcagtatgtcaattaaacgtggcggtgtctttactggacaatctgaacaggtt 
									gaacaccaagctaaagccgttgcaca 
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					Questions for students include: 
						 
						What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	How is swarming characterized in these bacteria? 
						2.	What is the function of the flagellar master operon flhDC? 
						 
						 
					 
					
					 
					 
					 
					 
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