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					Nucleotide Sequencing Results - CULTURE # 18 
						 
					 
					  Culture #18 - Set A 
					
						
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								 ctatttggggtgagtagataattgagccgcttttcaaactttttaatggtgaaaaaataatccatctcactcttgatgaagtaagacggatt 
									attgttgtttaaattagtactgtcttttaaagtgctttaaaaagagattgagcaggtatagtatttccttagtatttgttattctatgaaaatctct 
									agtttaaatcaatttatgtatgttattttcctattcaggtttatggttcaaatcattatctctaaaaaactagacattggagtaaaaggtatcat 
									ttatctgaatattgtaattatgatctcaaaaccgtagaaaatagtgaagatatgatttacctattttgtactgtttcttaatgttaattttgttccta 
									gtttagtaagtgttggtatttttcgaggtgctaataattgtttattgagaatatccatagctgttcgtcccatttcttctgtatagacagtgacact 
									agaaagtggaggatacacttgtttagctagagttgtatcgttaaaagagataatttgaatgtcgtcagggaccttgataccattttcctgaa 
									gtgctctgagtgcaccaatagctagactatcactagccgcaaagtaagcatcaggtaaagtagctccactcttaatcttagaatcaagaa 
									gttcatagccagattggacagtgaagtcaccagtcagaataaaaagagggtcatagattcccttttccatacagtagttccgataagaac 
									gtaaacgaggatcagagatgatttcagttgcatctgttgttttttcttgcccaatgagtaggcctatattattacaaccttgttctttaagataac 
									agagtgccgattgtacggagttttcaaaatcagtagtaacacatggatgtcct 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	When was the lactose operon of E. coli discovered? 
						2.	Where is the b-galactosidase gene of this bacteria located relative to the permease gene? 
						3.	Cultures of this bacteria do not usually ferment galactose.  Is this because they lack genes coding for enzymes that would allow galactose utilization to occur, or is it because these genes are not being induced?  
					 
					 
						 
					 
					
					  Culture #18 - Set B 
					
						
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								 ccaggatcctggcgagacactataagcaggcgctggaaggacaaatatcgaattgatgatggaattccatcatcaataaattgcttgtca 
									aaatagcgggagtcgcagataatccgctgcgacttatcactctcaaggaatcagaaatgataaaaagttcgtggcgtaaaattgcaatgc 
									tagccgccgttccgctgctgctggcgagcggcgcactgtgggccagtaccgatgctatccatcagaagctgacagatctcgagaagcgtt 
									caggcggcaggttgggcgtggcgctaatcaacacggcagataattctcaaatcttatatcgcggcgacgagcgttttgccatgtgcagcac 
									cagtaaagtgatggccgccgccgcggtattaaaacagagcgaaagcaataaagaggtggtaaataaaaggctggagattaacgcagc 
									cgatttggtggtctggagtccgattaccgaaaaacatctccagagcggaatgacgctggctgagctaagcgcggcgacgctgcaatatag 
									cgacaatacggcgatgaatctgatcatcggctaccttggcgggccggaaaaagtcaccgccttcgcccgcagtatcggcgatgccacctttc 
									gtctcgatcgtacggagcccacgctgaataccgccatcccgggcgatgagcgtgataccagcacgccgctggcgatggctgaaagcctac 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	Since the early 1980s these bacteria have been recognized as what? 
						2.	In wild type strains of these bacteria, chromosomal b-lactamases are constitutively produced at low levels and affect the organisms susceptibility to ___________________ while overproduction confers resistance to what? 
						 
					 
					
					 
					 
					 
					 
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