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					Nucleotide Sequencing Results - CULTURE # 17 
						 
					 
					  Culture #17 - Set A 
					
						
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								 atgaaaaaaacagcatttacattacttttattcattgccctaacgttgacaacaagtccacttgtaaatggtagcgagaaaagcgaagaaa 
									taaatgaaaaagatttgcgaaaaaagtctgaattgcagggaacagctttaggcaatcttaaacaaatctattattacaatgaaaaagcta 
									aaactgaaaataaagagagtcacgatcaatttttacagcatactatattgtttaaaggcttttttacagatcattcgtggtataacgatttatta 
									gtagattttgattcaaaggatattgttgataaatataaagggaaaaaagtagacttgtatggtgcttattatggttatcaatgtgcgggtggt 
									acaccaaacaaaacagcttgtatgtatggtggtgtaacgttacatgataataatcgattgaccgaagagaaaaaagtgccgatcaattta 
									tggctagacggtaaacaaaatacagtacctttggaaacggttaaaacgaataagaaaaatgtaactgttcaggagttggatcttcaagca 
									agacgttatttacaggaaaaatataatttatataactctgatgtttttgatgggaaggttcagaggggattaatcgtgtttcatacttctacagaa 
									ccttcggttaattacgatttatttggtgctcaaggacagtattcaaatacactattaagaatatatagagataataaaacgattaactctgaaa 
									acatgcatattgatatatatttatatacaagttaa 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						Note - Students must access the full-text article from Lancet to answer the questions. 
						1.	Describe the BLA Operon (name the genes present, what they code for, and what functions are associated with the proteins coded for). 
						2.	How do leukocidins kill phagocytic WBCs? 
						3.	What are superantigens? 
						 
						 
						 
					 
					
					  Culture #17 - Set B 
					
						
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								 atgatgaaaaaatcgatatgctgcgcactgctgctgacagcctctttctccacgtttgctgccgcaaaaacagaacaacaaattgccgatatcgtta 
									accgcaccatcacaccactgatgcaggagcaggctattccgggtatggccgtggcgattatctacgaggggaaaccttattactttacctggggtaa 
									agccgatatcgccaataaccacccagtcacgcagcaaacgctgtttgagctagggtcggtcagtaagacgtttaacggcgtgttgggcggcgacg 
									ctatcgcccgcggcgaaattaagctcagcgatccggtcacgaaatactggccagaactgacaggcaaacagtggcggggtatcagcctgctgca 
									cttagccacctatacagcgggtggcctgccgctgcagatccccgatgacgttacggataaagccgaattactgcgcttttatcaaaactggcaacca 
									caatggactccgggcgctaagcgtctttacgctaactccagcattggtctgtttggtgcgctggtggtaaaaccttcaggtatgagctacgaagaggc 
									aatgaccagacgcgtcctgcaaccattaaaactggcgcatacctggattacggttccgcaaagcgaacaaaaaaattatgcctggggctatcgcga 
									agggaagcctgtacacgtttctccgggacaacttgacgccgaagcctatggcgtgaaatccagcgttatcgatatggcccgctgggttcaggcca 
									acatggacgcc 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	Which antibiotics are the most widely used currently (accounting for 50% of the antibiotics used globally)? 
						2.	The Amp-C b-lactamases are primarily _________________ that mediate resistance to what? 
						 
						 
					 
					
					 
					 
					 
					 
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