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					Nucleotide Sequencing Results - CULTURE # 16 
						 
					 
					  Culture #16 - Set A 
					
						
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								 ggaagcatcagcttagcaatcccgcataccctgtctggcacctaccagatcttgtagttgagccggtacgagcgttctgtgttttatgca 
									atccacatcagcgaccagggatgctggctatttgaaacacttcacggaatgacgctgaaagtcttcgcgacctcgtctgtcgcacctta 
									acgaaagcattgcgaatccattaccgacaggtttccaaaagaaacccgggatgaaactcctattgcatttcgaaaattggaaacgac 
									aggcgaacatatgtaacgcgaaatttcaccctacgtataaacaatgcgcccagcgaatatcgctcccttaccgagcgacgaactcctgc 
									gcgccagcgaataaccggtgccgcgagggaaaagtttctccggcatacctggagagccctctcggaggcggcgcatgaacggtcag 
									cggtacagggaaacacccctcgacatcgagcgtctgcggcgcctgaatcgcgccacggtggagcgctacatggcaatgaagggggc 
									cgaacggttacagcggcacagcctgttcgtcgaggacggctgcgccggcaactggaccacggaaagcggcgaa 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Include abstract from NATURE (Medical significance of the organism being investigated) 
						 
						Find the article that provided the reference sequence and answer the following questions.   
						1.	What is pyocyanin? 
						2.	What are the pathophysiological effects of pyocyanin in human airways? 
						3.	What effect do the phenazines produced by these organisms have on alveolar macrophages?  
						 
					 
					 
						 
					 
					
					  Culture #16 - Set B 
					
						
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								 aagcttttgtgagccgtcgaagtgctcatgacaaggcgaagccgcagtcggggtgtatctccgcgttagcgagccgtaagggctgcttacgg 
									gcgtgttattcaataccttcagtgatatatcctacagcaactcaggactctagttacaacataatccatcacaggcaacgcgcctgcttttggctc 
									ccctaatacctgtgtcttcagccaagatgatgacagttatcggggggcattcaggttccgttaaatcccgttacctgttcctgaatgtttgaacgga 
									catgtcctgtctctgtttgttttcggaagcccgcatttcttccgaaaaaccatttttttgctacccgcctgactcaccacaagcaataagtctggaaac 
									ccgaggggcacagcatgctgcgcccctacgtattatcttctgccgcttctttcagtaaccattcgctaaatatttccatcgccggggtcattgctttcg 
									attttaaatgggttaaccagtaactgcccatattgatttcggtagcaaatgggcgcaccagctgcgcggtggttagctcccggacaaacattttcgc 
									tggggctaatgccacaccgccggaatggatagcgctctcgatcatcagacgggatgagtcaaagatggcgccattcactctcacagcctgcaa 
									gccagcggcagcaaaccagttttcccactcttcagcacggtaagaac 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1.	How have Gram-negative bacteria responded to the clinical use of extended-spectrum cephalosporins? 
						2.	Overproduction or constitutive production of inducible chromosomal b-lactamase causes what in these bacteria? 
						3.	These bacteria are known to gain resistance to b-lactams easily because of mutations in what? 
					 
					 
					 
					
					 
					 
					 
					 
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