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					Nucleotide Sequencing Results - CULTURE # 14 
						 
					 
					  Culture #14 - Set A 
					
						
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								 ggaagcatcagcttagcaatcccgcataccctgtctggcacctaccagatcttgtagttgagccggtacgagcgttctgtgttttatgcaatccaca 
									tcagcgaccagggatgctggctatttgaaacacttcacggaatgacgctgaaagtcttcgcgacctcgtctgtcgcaccttaacgaaagcattgcg 
									aatccattaccgacaggtttccaaaagaaacccgggatgaaactcctattgcatttcgaaaattggaaacgacaggcgaacatatgtaacgcg 
									aaatttcaccctacgtataaacaatgcgcccagcgaatatcgctcccttaccgagcgacgaactcctgcgcgccagcgaataaccggtgccgcg 
									agggaaaagtttctccggcatacctggagagccctctcggaggcggcgcatgaacggtcagcggtacagggaaacacccctcgacatcgagc 
									gtctgcggcgcctgaatcgcgccacggtggagcgctacatggcaatgaagggggccgaacggttacagcggcacagcctgttcgtcgaggac 
									ggctgcgccggcaactggaccacggaaagcggcgaa 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Include abstract from NATURE (Medical significance of the organism being investigated) 
						 
						Find the article that provided the reference sequence and answer the following questions. 
						1. What is pyocyanin? 
						2. What are the pathophysiological effects of pyocyanin in human airways? 
						3. What effect do the phenazines produced by these organisms have on alveolar macrophages? 
					 
						 
					 
					
					  Culture #14 - Set B 
					
						
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								 atggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtct 
									gtcttctggcttgcgtattaacagcgcgaaggatgacgccgcaggtcaggcgattgctaaccgttttacttctaacattaaaggcctgactcaggc 
									ggcccgtaacgccaacgacggtatttctgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtattcgtgaactg 
									acggttcaggccactacagggactaactccgattctgacctggactccatccaggacgaaatcaaatctcgtcttgatgaaattgaccgcgtatcc 
									ggccagacccagttcaacggcgtgaacgtgctggcgaaagacggttcaatgaaaattcaggttggtgcgaatgacggcgaaaccatcacgatc 
									gacctgaaaaaaatcgattctgatactctgggtctgaatggctttaacgtaaatggtaaaggtactattaccaacaaagctgcaacggtaagtga 
									tttaacttctgctggcgcgaagttaaacaccacgacaggtctttatgatctgaaaaccgaaaataccttgttaactaccgatgctgcattcgataaatt 
									agggaatggcgataaagtcacagttggcggcgtagattatacttacaacgctaaatctggtgattttactaccactaaatctactgctggtacgggtg 
									tagacgccgcggcgcaggctgctgattcagcttcaaaacgtgatgcgttagctgccacccttcatgctgatgtgggtaaatctgttaatggttcttacac 
									cacaaaagatggtactgtttctttcgaaacggat 
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					Questions for students include: 
					What is the name of the organism type from which this nucleotide sequence was taken? 
						What feature of the organism type is represented, i.e., what does this sequence code for? 
						 
						List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI). 
						 
						Find the article that provided the reference sequence, and answer the following questions. 
						1. What do genes located at the fliC locus code for in the subject bacteria? 
						2. Which antigens are most frequently used to identify different strains of these bacteria? 
						3. What type of disease is caused by these bacteria in the strain O157:H7 and how were these organisms transmitted to 477 U.S. residents in 1993? 
					 
					 
					
					 
					 
					 
					 
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