Bio. Sci. 4 - Microbiology Laboratory Syllabus
Harriet Wilson, Instructor
The laboratory syllabus included here is being compiled in sections this semester. This document (currently sold in the campus bookstore) is being revised and converted into electronic files. The laboratory syllabus is intended to provide students with electronic access to the information necessary for completing the laboratory portion of Sierra College microbiology classes (Bio. Sci. 4 and 8A-8B). Laboratory Syllabus - Copyright 2016 Harriet S. Wilson. All Rights Reserved.
Laboratory Syllabus Sections (PDF):
Laboratory Syllabus Cover - Fall 2016
Title Page
Introduction to Microbiology
Laboratory Procedures and Safety Regulations
Suggested Study Techniques
Semester Project
Significance of Handwashing
Microscopy, Microscopic Measurement & Observation/scientific Illustration
Culture of Microorganisms and Media Preparation
Aseptic Technique, Streak Plates and Pure Culture Technique
Staining - Direct and Indirect Stains
Staining - Gram stain and KOH Test
Staining - Acid-Fast stain
Staining - Endospore, Capsule and Flagellar Stains
Morphological Unknown
Introduction to Bacteria and Cyanobacteria
Introduction to Fungi
Introduction to Algae and Protozoa
Introduction to Multicellular Parasites
Food Microbiology - Fermented and Cultured Foods
Study Guide for Laboratory Exam 1
Determination of Microbial Numbers
Use of Selective and Differential Media and Replica Plating
The Scientific Method and Microbial ID Supplements
Charbohydrate Metabolism, Catalase, and Oxidase Tests
Lysine Decarboxylation, Citrate Utilization, Coagulase and CAMP Test
Gelatin Liquefaction, TSI, SIM, Urea Hydrolysis and Nitrate Reduction
Application of the PCR in Bacterial Identification
Nucleotide Sequencing, Electropherogram Evaluation and Bioinformatics
The Mini-screen, Rapid Isolation of Plasmid DNA
Gel Electrophoresis of DNA Samples
Restriction Endonuclease Digestion of DNA and RFLP
Competent Cell Preparation and Transformation
Introduction to Viruses, Phage Typing and Isolation of Coliphage
Bacteriophage Reproduction and Plaque Formation
Microbial Control (Effects of Temperature, UV Light, Disinfectants and Antiseptics)
Antimicrobial Sensitivity Testing
Bacteriological Examination of Water
Composition of Blood and White Cell Study
Diagnostic Immunology (Precipitation, Agglutination and ELISA)
Appendix A and B and References
Answers to Laboratory Exercise Questions
Bio. Sci. Recommended Study Techniques (Web Version)
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