Sierra College Department of Biological Sciences

Bio. Sci. 4 - Microbiology Laboratory Syllabus

Harriet Wilson, Instructor

The laboratory syllabus included here is being compiled in sections this semester. This document (currently sold in the campus bookstore) is being revised and converted into electronic files. The laboratory syllabus is intended to provide students with electronic access to the information necessary for completing the laboratory portion of Sierra College microbiology classes (Bio. Sci. 4 and 8A-8B). Laboratory Syllabus - Copyright 2016 Harriet S. Wilson. All Rights Reserved.

Laboratory Syllabus Sections (PDF):


• Laboratory Syllabus Cover - Fall 2016

• Title Page

• Introduction to Microbiology

• Laboratory Procedures and Safety Regulations

• Suggested Study Techniques

• Semester Project

• Significance of Handwashing

•Microscopy, Microscopic Measurement & Observation/scientific Illustration

•Culture of Microorganisms and Media Preparation

•Aseptic Technique, Streak Plates and Pure Culture Technique

•Staining - Direct and Indirect Stains

•Staining - Gram stain and KOH Test

•Staining - Acid-Fast stain

•Staining - Endospore, Capsule and Flagellar Stains

•Morphological Unknown

•Introduction to Bacteria and Cyanobacteria

•Introduction to Fungi

•Introduction to Algae and Protozoa

•Introduction to Multicellular Parasites

•Food Microbiology - Fermented and Cultured Foods

•Study Guide for Laboratory Exam 1

•Determination of Microbial Numbers

•Use of Selective and Differential Media and Replica Plating

•The Scientific Method and Microbial ID Supplements

•Charbohydrate Metabolism, Catalase, and Oxidase Tests

• Lysine Decarboxylation, Citrate Utilization, Coagulase and CAMP Test

•Gelatin Liquefaction, TSI, SIM, Urea Hydrolysis and Nitrate Reduction

•Application of the PCR in Bacterial Identification

•Nucleotide Sequencing, Electropherogram Evaluation and Bioinformatics

•The Mini-screen, Rapid Isolation of Plasmid DNA

•Gel Electrophoresis of DNA Samples

•Restriction Endonuclease Digestion of DNA and RFLP

•Competent Cell Preparation and Transformation

•Introduction to Viruses, Phage Typing and Isolation of Coliphage

•Bacteriophage Reproduction and Plaque Formation

•Microbial Control (Effects of Temperature, UV Light, Disinfectants and Antiseptics)

•Antimicrobial Sensitivity Testing

•Bacteriological Examination of Water

•Composition of Blood and White Cell Study

•Diagnostic Immunology (Precipitation, Agglutination and ELISA)

•Appendix A and B and References

•Answers to Laboratory Exercise Questions

Bio. Sci. Recommended Study Techniques (Web Version)