Simulated RFLP Gels
Carefully Prepared for
Bio. Sci. 4 Microbiology Students,
Harriet Wilson's Classes
Spring 2016
RFLP 1 Gel #1 containing samples 1-5 in lanes as numbered. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 = 582, 472, 209, 115, 95, 31
Lane #2 = 428, 352, 266, 231, 86, 74, 33
Lane #3 = 310, 286, 265, 209, 207, 165, 73
Lane #4 = 572, 374, 207, 98, 86, 79, 51, 42
Lane #5 = 526, 394, 286, 204, 79
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
RFLP 2 Gel #2 containing samples 6-10 in lanes 1-5. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 (sample #6) = 540, 264, 209, 202, 164, 50, 42, 31
Lane #2 (sample #7) = 475, 325, 286, 206, 102, 49, 27, 21
Lane #3 (sample #8) = 359, 328, 209, 179, 128, 79, 42, 33, 31
Lane #4 (sample #9) = 546, 449, 209, 207, 42
Lane #5 (sample #10) = 481, 286, 214, 206, 205, 104
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
RFLP 3 Gel #3 containing samples 11-15 in lanes 1-5. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 (sample #11) = 430, 264, 246, 207, 162, 145, 63
Lane #2 (sample #12) = 475, 310, 286, 205, 165, 48
Lane #3 (sample #13) = 571, 368, 209, 115, 98, 95, 42
Lane #4 (sample #14) = 311, 286, 268, 215, 209, 206
Lane #5 (sample #15) = 211, 207, 195, 187, 180, 174, 166, 98, 42
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
RFLP 4 Gel #4 containing samples 16-20 in lanes 1-5. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 (sample #16) = 473, 428, 209, 186, 173, 48
Lane #2 (sample #17) = 636, 387, 186, 113, 83, 60, 48
Lane #3 (sample #18) = 475, 371, 207, 144, 118, 65, 42, 31
Lane #4 (sample #19) = 310, 286, 268, 209, 206, 173, 35
Lane #5 (sample #20) = 572, 375, 207, 98, 85, 79, 48, 42
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
RFLP 5 Gel #5 containing samples 21 in lane #5. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 (no sample)
Lane #2 (no sample)
Lane #3 (no sample)
Lane #4 (no sample)
Lane #5 (sample #21) = 599, 359, 222, 209, 106
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
Copyright 2012 Sierra College Biological Sciences Department. All Rights Reserved.
