Simulated RFLP Gels
Carefully Prepared for
Bio. Sci. 4 Microbiology Students,
Harriet Wilson's Classes
Fall 2015
RFLP 1 Gel #1 containing samples 1-5 in lanes as numbered. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 = 361, 264, 210, 209, 202, 124, 103, 23
Lane #2 = 311, 286, 266, 209, 207, 166, 48
Lane #3 = 577, 266, 217, 164, 86, 77, 42, 33, 14
Lane #4 = 371, 211, 207, 186, 166, 144, 97, 42, 32
Lane #5 = 824, 265, 208, 84, 50, 44, 38, 5
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
RFLP 2 Gel #2 containing samples 6-10 in lanes 1-5. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 (sample #6) = 599, 209, 207, 162, 162, 102, 48, 15
Lane #2 (sample #7) = 310, 285, 227, 223, 212, 205
Lane #3 (sample #8) = 395, 310, 286, 204, 150, 79, 46, 15
Lane #4 (sample #9) = 613, 263, 207, 207, 131, 79
Lane #5 (sample #10) = 601, 359, 209, 90, 80, 79, 68, 16
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
RFLP 3 Gel #3 containing samples 11-15 in lanes 1-5. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 (sample #11) = 263, 212, 209, 207, 159, 144, 120, 110, 32
Lane #2 (sample #12) = 429, 214, 209, 186, 177, 123, 84, 74, 51
Lane #3 (sample #13) = 361, 211, 207, 195, 180, 166, 98, 52, 42
Lane #4 (sample #14) = 328, 242, 202, 172, 145, 121, 98, 87, 47, 32, 30
Lane #5 (sample #15) = 429, 265, 207, 207, 186, 90, 86, 53
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
RFLP 4 Gel #4 containing samples 16-20 in lanes 1-5. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 (sample #16) = 376, 217, 212, 207, 165, 144, 96, 51, 42
Lane #2 (sample #17) = 615, 264, 209, 207, 174, 49
Lane #3 (sample #18) = 419, 402, 216, 186, 88, 87, 72, 50
Lane #4 (sample #19) = 310, 286, 267, 209, 206, 170, 74
Lane #5 (sample #20) = 373, 282, 207, 199, 191, 93, 69, 48, 42
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
RFLP 5 Gel #5 containing samples 21-25 in lanes 1-5. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 (sample #21) = 475, 286, 254, 223, 206, 48
Lane #2 (sample #22) = 571, 211, 209, 207, 202, 58, 42
Lane #3 (sample #23) = 475, 310, 286, 206, 165, 48
Lane #4 (sample #24) = 600, 451, 224, 144, 9
Lane #5 (sample #25) = 615, 471, 209, 104, 86, 42
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
Copyright 2012 Sierra College Biological Sciences Department. All Rights Reserved.
