Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 9

• Culture #9 - Set A

gaattccgcctttctttgctttttgattattatacaaaaaaaaagacggtgttcaaaagaaaaactcatgaaaaaacgcctcagctttcactgaa
acgcctttttttatttatctttaataaaaatagcaatgatgtcaaaaaatagtcgattttactaagtgatgaattatgggtggtacaaatactgctgg
aaatagttgttaacttcaagggcatcttctttattttttgaaagaattaagatggtatcatccccagcgatgcagcccaagatatgagggttgtgg
atacgtatgcgatcaattaaaaccccaatggcttgcccgttgccaggtaagacggttaaaagatttgtgaactctatttgtgttaaagaaacacca
gtttcaataataatgtcaacaagacgttcttcatctgttttatattttggacgaagggcagaattattcaagatacgataataggcttctccttgacca
ttgtctactttgtttaaattcagctcacgaatgtcaccagaaatagttgcttgtgtggtgtgaatattttctttttccaataatgctagtaattcactttgtg
tttggatttcattttctgtgaccaatcgcttaatcatcgtttggcgttttaatcggttcattactttcctccagttcagttaatcatgcaaaaaaacatcatt
gctgttatttaaaacggtgccagtaattccctaaaatcactgtacaacgttttactaaattgtgcaagtctcacttttttaaatgcgaaatatccgaca
aaatcaatgagaaccttttagctaaagcaccctttttctattctaattagagagattctgctagtattgctttgtaaatgtctggaggaagggtgtta
tttgtgaataagc

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?
List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. What is carbamate kinase and what does it do?
2. The gene for this enzyme in this bacteria is identical in nucleotide sequence to a gene from what other type of bacteria?
3. What other enzyme has been found to have a similar structure and perhaps share a common catalytic mechanism with this one?



• Culture #9 - Set B

gaggccgatagtctggaaaaactcgaagccctgctaccctggaacgtcccgctggagcccattgcaaaaaaagtggcgcaatacaacgaa
ggcaagtaggtcgatttagcggcgcttacgggagaacagcttgccgggcttgcatcggccatgcgctaatcctgtggcataaccataagggg
ataaacaatgtggcagtggcttggaaaagtgcggtggttgctggtggtgatagcgctgctgggtggcaaggtcatggcggcgtctgaactgg
atagcgcgctggccagactcgagcagcagcatcatgggcgtctggggctggcctatatcgatagtggcagcggcgagagttacagctatcgc
ggtgaggagcgttttgccttttgcagcaccttcaaggcggtgctggcgggggcggtgctgcagcgaagcgtcagtcagcccggtttgctcgata
agcgggtgcattatgcggccacggatctgctcgcctatgcccccatcaccaagacgcatctggataaagggatgcgcatcggtgaactggccg
cagcggcggtgcaatatagtgacaacacggcggccaacctgctgctgcaagaaattggcggcgtccaggcgctgaaccggtttgtgcaggg
gttgggagatccggcattccgtctggataggatagagcctcaccttaatagtgccgagccaggagatgtgcgcgataccaccacgccgctggc
catggcccacaccctgcaggccatgactctgggcaaggggtta

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. Where were ESBLs first characterized for members of the family Enterobacteriaceae?
2. What are ESBLs and what do these enzymes do?



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