Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 8

• Culture #8 - Set A

atgaaaaaaacagcatttacattacttttattcattgccctaacgttgacaacaagtccacttgtaaatggtagcgagaaaagcgaagaa
ataaatgaaaaagatttgcgaaaaaagtctgaattgcagggaacagctttaggcaatcttaaacaaatctattattacaatgaaaaagct
aaaactgaaaataaagagagtcacgatcaatttttacagcatactatattgtttaaaggcttttttacagatcattcgtggtataacgatttat
tagtagattttgattcaaaggatattgttgataaatataaagggaaaaaagtagacttgtatggtgcttattatggttatcaatgtgcgggtgg
tacaccaaacaaaacagcttgtatgtatggtggtgtaacgttacatgataataatcgattgaccgaagagaaaaaagtgccgatcaatttat
ggctagacggtaaacaaaatacagtacctttggaaacggttaaaacgaataagaaaaatgtaactgttcaggagttggatcttcaagcaa
gacgttatttacaggaaaaatataatttatataactctgatgtttttgatgggaaggttcagaggggattaatcgtgtttcatacttctacagaa
ccttcggttaattacgatttatttggtgctcaaggacagtattcaaatacactattaagaatatatagagataataaaacgattaactctgaaa
acatgcatattgatatatatttatatacaagttaa

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
Note - Students must access the full-text article from Lancet to answer the questions.
1. Describe the BLA Operon (name the genes present, what they code for, and what functions are associated with the proteins coded for).
2. How do leukocidins kill phagocytic WBCs?
3. What are superantigens?


• Culture #8 - Set B

ttgtgaaaggtaaacgttcattacatgtgtgtaatgccccatcacctgctgcaacagcgtctattgagataggacgagaaatagtaaaagagcattt
ttctttgtaattgaaagacgatactgcgagtaaaaataataaatagcactgaaactcatcagtgctttttatatcactcacaacactcttttagtgcttca
tttattagccactgctgaaatatttccattgaggcggtcattggttttgactttaaatacgttaaccaatacttacccatttcaacttctattttaaagggctg
tactaactgtccattcttaatttctcttgaaaacatttttgctggagctaatgcaactcccccttcataaattgcactttcaatcattaaacgtgaggagtca
aaaatagaacccgttatttttatcggtgatatttctgctttttcaaaccattttaaccactcatcttctcgatatgagcgatataaattttcatttattaagtcag
ttggataatgtagtcgttttgctgttgttggtgagcacaagaccgttaatggtgcagagaataaggctttattatgagttaaaggccataacccttcacca
aatcgaatagcaaaatccaatccttcggtagctaaattaaccacattattattggttctcaaatttacttctattcttggatagagttgcctgaattcagcca
atctgggtaataaccatcccacagcgaatgtgccgacagccgcaatggaaaccatcaccacatt

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. The organisms featured in this article were previously identified as what? (Note - The previous name applies to your unknown).
2. These organisms are naturally resistant to what?
3. According to the French Society for Microbiology (see case report), the pattern of synergy observed with the antimicrobial susceptibility testing was due to what?




W3C Logo: Valid HTML 4.01 Transitional W3C Logo: Valid CSS