Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 4

• Culture #4 - Set A

tagctttgtacgatagtacttaattaatgaaccaatcttcattttcataacctcccgtatcgaatgactgtattttacatttattataatcataattttc
ccaataatcaaaaaacctaaattatggtagattcaaattgtagtactataacttaatttgaaaaggagttggaaagaatgaaaaaaatgaa
aagtacgttgctaagtcttttgattgcaggatcagtaataaacccggtgtacgcaacagcgatggatagcgaagatagcttaggaaacgac
ggtggtcaggagaaattccgtgtttatgttgaggcaaacaaaaaaacagaaaaatcaacattaaaaactcaatattctgctcgttgggaatt
aacagagaacggattttcaactgatatgaatgaaaaacaattcgaagcattacagaagaataaaaatttcacagttacaaaagtgcctatcg
ttacattagatacgataggtttggaagacgatagtatggtagaaaatgaaactctggatttggtaacttctttaagagcttctcaacaaatacct
tggggcattaaagcaatttataacaatgatactttaacatctacaactggaggaagtggcattaatattgccgtactcgatacgggtgtaaata
cttctcatcctgaccttgtcaataacgtagagcaatgtaaagactttacaggtgcgacaacacccattaataacagttgtactgatagaaacgg
acatggtacacatgttgctggtacggccttagctgatggtggaagcgatcaagcaggcatttacggtgtagctccagatgctgatctttgg

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?
List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. These bacteria have been used along with B. thuringiensis to control what?
2. Why is it desireable to combine multiple toxin genes in a single population of bacteria?
3. Why are these organisms considered to be the best host for combining multiple toxin genes?



• Culture #4 - Set B

ttgtgaatcagcaaaacgccgggttattcttatttgtcgcttctttactcgcctttatcggccctcactcaaggatgtattgtggttatgcgttattt
tcgcctgtgtattatctccctgttagccaccctgccgctggcggtacacgccagcccgcagccgcttgagcaaattaaactaagcgaaagc
cagctgtcgggcagcgtaggcatgatagaaatggatctggccagcggccgcacgctgaccgcctggcgcgccgatgaacgctttcccat
gatgagcacctttaaagtagtgctctgcggcgcagtgctggcgcgggtggatgccggtgacgaacagctggagcgaaagatccactatc
gccagcaggatctggtggactactcgccggtcagcgaaaaacaccttgccgacggcatgacggtcggcgaactctgtgccgccgccatta
ccatgagcgataacagcgccgccaatctgctgctggccaccgtcggcggccccgcaggattgactgcctttttgcgccagatcggcgacaa
cgtcacccgccttgaccgctgggaaacggaactgaatgaggcgcttcccggcgacgcccgcgacaccactaccccggccagcatggccg
cgaccctgcgcaagctgctgaccagccagcgtctgagcgcccgttcgcaacggcagctgctgcagtggatggtggacgatcgggtcgccg
gaccgttgatccgctccgtgctgccggcgggctggtttatcgccgataagaccggagctgccaaacggggtgcgcgcgggattgtcgccctg
cttggcccgaataacaaagcagagcggattgtggtgatttatctgcgggatacgccggcgagcatggccgagcgaaatcag

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. What are ESBLs and what is their significance?
2. The bacteria that carry genes coding for ESBLs are frequently associated with what types of infections?



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