Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 3

• Culture #3 - Set A

tggagatacggctctttctgcaaatgaagcgagaatgaaagagaccttgcaaaaggctggattatttgcaaaatctatgaatgcctattcttatatgt
taattaagaatcctgatgtaaattttgaaggaattactattaatggatatgtagatttacctggtagaatcgtacaagatcaaaagaatgcaagagca
catgctgttacttgggacacgaaagtaaaaaaacagcttttagatacattgactggtattgttgaatatgataccacgttcgacaattattatgaaac
attggttgaggcgattaatacaggggatggagaaactttaaaagaaggaattacagatttacgaggtgaaattcaacaaaaccaaaagtacgca
caacaattaatagaagaattaactaaattaagagactctattggacaagatgttagagcatttggaggcaataaagatcttttgcaatcgattctaaa
aaaccaaggtgcagatgttgatgcagatcaaaagcgtctagaagaagtattaggatcagtaaactattataaac

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. What four species belong within this group of bacteria?
2. Which newly described species is psychroduric?
3. What symptoms are associated with the emetic toxin and enterotoxins produced by these bacteria?


• Culture #3 - Set B

atggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcg
tctgtcttctggcttgcgtattaacagcgcgaaggatgacgccgcaggtcaggcgattgctaaccgttttacttctaacattaaaggcctgactc
aggcggcccgtaacgccaacgacggtatttctgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtattcg
tgaactgacggttcaggccactacagggactaactccgattctgacctggactccatccaggacgaaatcaaatctcgtcttgatgaaattga
ccgcgtatccggccagacccagttcaacggcgtgaacgtgctggcgaaagacggttcaatgaaaattcaggttggtgcgaatgacggcgaa
accatcacgatcgacctgaaaaaaatcgattctgatactctgggtctgaatggctttaacgtaaatggtaaaggtactattaccaacaaagct
gcaacggtaagtgatttaacttctgctggcgcgaagttaaacaccacgacaggtctttatgatctgaaaaccgaaaataccttgttaactaccg
atgctgcattcgataaattagggaatggcgataaagtcacagttggcggcgtagattatacttacaacgctaaatctggtgattttactaccacta
aatctactgctggtacgggtgtagacgccgcggcgcaggctgctgattcagcttcaaaacgtgatgcgttagctgccacccttcatgctgatgtg
ggtaaatctgttaatggttcttacaccacaaaagatggtactgtttctttcgaaacggat

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. What do genes located at the fliC locus code for in the subject bacteria?
2. Which antigens are most frequently used to identify different strains of these bacteria?
3. What type of disease is caused by these bacteria in the strain O157:H7 and how were these organisms transmitted to 477 U.S. residents in 1993?




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