Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 26

• Culture #26 - Set A

tagctttgtacgatagtacttaattaatgaaccaatcttcattttcataacctcccgtatcgaatgactgtattttacatttattataatcata
attttcccaataatcaaaaaacctaaattatggtagattcaaattgtagtactataacttaatttgaaaaggagttggaaagaatgaaa
aaaatgaaaagtacgttgctaagtcttttgattgcaggatcagtaataaacccggtgtacgcaacagcgatggatagcgaagatagc
ttaggaaacgacggtggtcaggagaaattccgtgtttatgttgaggcaaacaaaaaaacagaaaaatcaacattaaaaactcaatat
tctgctcgttgggaattaacagagaacggattttcaactgatatgaatgaaaaacaattcgaagcattacagaagaataaaaatttca
cagttacaaaagtgcctatcgttacattagatacgataggtttggaagacgatagtatggtagaaaatgaaactctggatttggtaactt
ctttaagagcttctcaacaaataccttggggcattaaagcaatttataacaatgatactttaacatctacaactggaggaagtggcattaa
tattgccgtactcgatacgggtgtaaatacttctcatcctgaccttgtcaataacgtagagcaatgtaaagactttacaggtgcgacaaca
cccattaataacagttgtactgatagaaacggacatggtacacatgttgctggtacggccttagctgatggtggaagcgatcaagcagg
catttacggtgtagctccagatgctgatctttgg

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?
List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. These bacteria have been used along with B. thuringiensis to control what?
2. Why is it desireable to combine multiple toxin genes in a single population of bacteria?
3. Why are these organisms considered to be the best host for combining multiple toxin genes?



• Culture #26 - Set B

ccaggatcctggcgagacactataagcaggcgctggaaggacaaatatcgaattgatgatggaattccatcatcaataaattgct
tgtcaaaatagcgggagtcgcagataatccgctgcgacttatcactctcaaggaatcagaaatgataaaaagttcgtggcgtaaa
attgcaatgctagccgccgttccgctgctgctggcgagcggcgcactgtgggccagtaccgatgctatccatcagaagctgacag
atctcgagaagcgttcaggcggcaggttgggcgtggcgctaatcaacacggcagataattctcaaatcttatatcgcggcgacga
gcgttttgccatgtgcagcaccagtaaagtgatggccgccgccgcggtattaaaacagagcgaaagcaataaagaggtggtaaa
taaaaggctggagattaacgcagccgatttggtggtctggagtccgattaccgaaaaacatctccagagcggaatgacgctggct
gagctaagcgcggcgacgctgcaatatagcgacaatacggcgatgaatctgatcatcggctaccttggcgggccggaaaaagtc
accgccttcgcccgcagtatcggcgatgccacctttcgtctcgatcgtacggagcccacgctgaataccgccatcccgggcgatgag
cgtgataccagcacgccgctggcgatggctgaaagcctac

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. Since the early 1980s these bacteria have been recognized as what?
2. In wild type strains of these bacteria, chromosomal b-lactamases are constitutively produced at low levels and affect the organisms susceptibility to ___________________ while overproduction confers resistance to what?




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