Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 25

• Culture #25 - Set A

acaatatcagatatgaaacataaagtgttaggagcacaatcagcttcatcaggttatgactccttgttaagaactcctaaactgctgaaaga
ttttattaaaaataaagacgctaatcaatatgaaacctttacacaagcttttattgatttaaaatcagatcgtatcgatggaatattgattgacaa
agtatatgccaattactatttagcaaaagaagggcaattagagaattatcggatgatcccaacgacctttgaaaatgaagcattttcggttgg
acttagaaaagaagacaaaacgttgcaagcaaaaattaatcgtgctttcagggtgctttatcaaaatggcaaatttcaagctatttctgagaa
atggtttggagatgatgttgccactgccaatattaaatcttaaaaaggacacttgtcctttttttgtttaacaggtgagatctagcagacaaagtc
agctagacgatttaataaaaatcttagtttaaggttttttattaaactgtattttttctgaaaattatag

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence and answer the following questions.
1. What is the CAMP reaction?
2. What is the name of the bacteria that produce sphingomyalinase C (beta toxin)?



• Culture #25 - Set B

ttgtgaatcagcaaaacgccgggttattcttatttgtcgcttctttactcgcctttatcggccctcactcaaggatgtattgtggttatgcgtta
ttttcgcctgtgtattatctccctgttagccaccctgccgctggcggtacacgccagcccgcagccgcttgagcaaattaaactaagcgaa
agccagctgtcgggcagcgtaggcatgatagaaatggatctggccagcggccgcacgctgaccgcctggcgcgccgatgaacgcttt
cccatgatgagcacctttaaagtagtgctctgcggcgcagtgctggcgcgggtggatgccggtgacgaacagctggagcgaaagatc
cactatcgccagcaggatctggtggactactcgccggtcagcgaaaaacaccttgccgacggcatgacggtcggcgaactctgtgccg
ccgccattaccatgagcgataacagcgccgccaatctgctgctggccaccgtcggcggccccgcaggattgactgcctttttgcgccag
atcggcgacaacgtcacccgccttgaccgctgggaaacggaactgaatgaggcgcttcccggcgacgcccgcgacaccactaccccg
gccagcatggccgcgaccctgcgcaagctgctgaccagccagcgtctgagcgcccgttcgcaacggcagctgctgcagtggatggtgg
acgatcgggtcgccggaccgttgatccgctccgtgctgccggcgggctggtttatcgccgataagaccggagctgccaaacggggtgcg
cgcgggattgtcgccctgcttggcccgaataacaaagcagagcggattgtggtgatttatctgcgggatacgccggcgagcatgg
ccgagcgaaatcag

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. What are ESBLs and what is their significance?
2. The bacteria that carry genes coding for ESBLs are frequently associated with what types of infections?




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