Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 24

• Culture #24 - Set A

atgaaaaaatttatacttcctatattcagcatttctattctagtttctctcagtgcatgttcatctattaaaactaaatctgaagataattttcatattt
cttctcagcaacatgaaaaagctattaaaagctattttgatgaagctcaaacacagggtgtaattattattaaagagggtaaaaatcttagc
acctatggtaatgctcttgcacgagcaaataaagaatatgtccctgcatcaacatttaagatgctaaatgctttaatcgggctagaaaatcat
aaagcaacaacaaatgagattttcaaatgggatggtaaaaaaagaacttatcctatgtgggagaaagatatgactttaggtgaggcaatg
gcattgtcagcagttccagtatatcaagagcttgcaagacggactggcctagagctaatgcagaaagaagtaaagcgggttaattttggaa
atacaaatattggaacacaggtcgataatttttggttagttggcccccttaaaattacaccagtacaagaagttaattttgccgatgaccttgca
cataaccgattaccttttaaattagaaactcaagaagaagttaaaaaaatgcttctaattaaagaagtaaatggtagtaagatttatgcaaaa
agtggatggggaatgggtgttactccacaggtaggttggttgactggttgggtggagcaagctaatggaaaaaaaatccccttttcgctcaac
ttagaaatgaaagaaggaatgtctggttctattcgtaatgaaattacttataagtcgctagaaaatcttggaatcatttaa

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. What types of diseases are caused by these bacteria?
2. What antimicrobial agents have recently become the drugs of choice for controlling these bacteria, and what has occurred to present a problem with this practice?

3. The bacteria that served as the subject of this research paper came from what source?



• Culture #24 - Set B

atggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcg
tctgtcttctggcttgcgtattaacagcgcgaaggatgacgccgcaggtcaggcgattgctaaccgttttacttctaacattaaaggcctgact
caggcggcccgtaacgccaacgacggtatttctgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtatt
cgtgaactgacggttcaggccactacagggactaactccgattctgacctggactccatccaggacgaaatcaaatctcgtcttgatgaaatt
gaccgcgtatccggccagacccagttcaacggcgtgaacgtgctggcgaaagacggttcaatgaaaattcaggttggtgcgaatgacgg
cgaaaccatcacgatcgacctgaaaaaaatcgattctgatactctgggtctgaatggctttaacgtaaatggtaaaggtactattaccaaca
aagctgcaacggtaagtgatttaacttctgctggcgcgaagttaaacaccacgacaggtctttatgatctgaaaaccgaaaataccttgttaa
ctaccgatgctgcattcgataaattagggaatggcgataaagtcacagttggcggcgtagattatacttacaacgctaaatctggtgattttact
accactaaatctactgctggtacgggtgtagacgccgcggcgcaggctgctgattcagcttcaaaacgtgatgcgttagctgccacccttcatg
ctgatgtgggtaaatctgttaatggttcttacaccacaaaagatggtactgtttctttcgaaacggat

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. What do genes located at the fliC locus code for in the subject bacteria?
2. Which antigens are most frequently used to identify different strains of these bacteria?
3. What type of disease is caused by these bacteria in the strain O157:H7 and how were these organisms transmitted to 477 U.S. residents in 1993?




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