Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 22

• Culture #22 - Set A

aagcttaattatcaataatgaactaggtaacatactgtccataaaagtacacttcaagaaaaatagtgcacttagacgtaagaagttta
cttggagagatgacgctaatagtaagaagacgagtggatcattgggggatctgggtattcaccttattgacatggtatggtatttgttcga
gagtgatttcatcacagaatcagtaagggcaaagatgaacacaaatgtaaaaacaaaagaggataaacaggtacttgtagatgact
atgcagaaatttatggccagctgaagaacaaggtatttgtaaatatcatcacatcaaagtgttctgtacctgaagactgtggttttagcatt
gaggtagttggacacaaaaaagagtttaaataccacacaggtaatcctcacgtttacaagctcatagatggcttgaacgtggtagactg
cccagtaccgcaaagcctattaaacgatccgccaaacgagttttatggatgggctgattcttttagaagcgagttaatcaattggattgca
tcaactcagaatgattgggttgagatcccttcttttagtgatggttttagatctcaggaagtattagaaatgttctttgagaaagacagcaac
tctcaacccatgtctgtttcagcagtcaactagtatttcaaagagagaagttactaaaaaagcaggaatttactttcctgctttttcatatagg
ggtgtaatgaatgttaagagggacatatttatttggatatgctttcttttttacagtaggtattatccatatatcaacagggagtttgacacca
tttttattagaggcttttaac

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?
List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. Catabolite repression in these organisms involves a catabolite control protein A (CcpA) which interacts with __________________________________________________________ to effect catabolite repression by a negative control mechanism.
2. What is the product of the newly identified gene glcP, and what is the function of this protein?
3. The mutant bacteria presented in this paper did not display glucose mediated catabolite repression, but they exhibited strong repression in response to what? (See discussion section.)




• Culture #22 - Set B

gcggagtacatcgcgttctgcacgcgctccggctgcgtctgcagcgagaccaattggctgttgtaggtcgcaagatcgttctgcgcgctttgcag
atcgtcgagcgcctgagacaccctggattcaagctgtcgcaacgataacgtgctcagggttttacgggtgacctcatcatcaggaacgtcgctca
gcgcattcaggctgtctaccgcctgacgcattttcgccggcgcctgttcaacctgctgacgcagttgcacagtttcagactttatacgctcaatctt
atcgagcgtttctaaagtctgcgtcaggtcctgctgaactaatttttcctgcggcgtgagttctttttgcttatttagcgtagtgagctggctttgaatgt
cagaacggtcaggtaaatctgcagcctgaacctggctgaagctcagagtgccaactataaggaccagtgcaaagacaatggtggaaatagc
atgaggccaacgatttgtatgctgcatagtattaacatgaatggttgcaaatgccggagaataccgggggtcgtcgcagcgcgcagaatagca
cttctccctgcgcgcgaatagcggcagagataagtaaaatttacctttatttacgcctgcggattcaggccatgacctcagacgaacttcgtagcg
acgggcaggtctgatacatctcaatcagcgcatcgacataagcacgggcttc

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. For the last decade, these commensal Gram-negative bacteria have been rapidly emerging as what?
2. The pumps used by these bacteria to remove disinfectants and antibiotics from their cytoplasm (cytosol) use what as a source of energy?




W3C Logo: Valid HTML 4.01 Transitional W3C Logo: Valid CSS