Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 21

• Culture #21 - Set A

gaattccgcctttctttgctttttgattattatacaaaaaaaaagacggtgttcaaaagaaaaactcatgaaaaaacgcctcagctttcactg
aaacgcctttttttatttatctttaataaaaatagcaatgatgtcaaaaaatagtcgattttactaagtgatgaattatgggtggtacaaatact
gctggaaatagttgttaacttcaagggcatcttctttattttttgaaagaattaagatggtatcatccccagcgatgcagcccaagatatgagg
gttgtggatacgtatgcgatcaattaaaaccccaatggcttgcccgttgccaggtaagacggttaaaagatttgtgaactctatttgtgttaaa
gaaacaccagtttcaataataatgtcaacaagacgttcttcatctgttttatattttggacgaagggcagaattattcaagatacgataatag
gcttctccttgaccattgtctactttgtttaaattcagctcacgaatgtcaccagaaatagttgcttgtgtggtgtgaatattttctttttccaataat
gctagtaattcactttgtgtttggatttcattttctgtgaccaatcgcttaatcatcgtttggcgttttaatcggttcattactttcctccagttcagtta
atcatgcaaaaaaacatcattgctgttatttaaaacggtgccagtaattccctaaaatcactgtacaacgttttactaaattgtgcaagtctca
cttttttaaatgcgaaatatccgacaaaatcaatgagaaccttttagctaaagcaccctttttctattctaattagagagattctgctagtattgc
tttgtaaatgtctggaggaagggtgttatttgtgaataagc

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?
List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. What is carbamate kinase and what does it do?
2. The gene for this enzyme in this bacteria is identical in nucleotide sequence to a gene from what other type of bacteria?
3. What other enzyme has been found to have a similar structure and perhaps share a common catalytic mechanism with this one?




• Culture #21 - Set B

tctagatacaccatatggcctagcaacatcagatacaggaatagaaccatctttgagtatgccctaatctccctgatatcctttttctg
taactttggcttccgcccacccaagcgaccgcgagctcttgcggcttccaatcccgccttggtacgttcgctaatcaaatttcgctcaa
attcagccaaagcagcaaacacatgaaaaaccagctttccagaagcactgttcgtttcaatctgttcttgtaggctcagaaatccaa
tccgtcgctcatcaaccagatgggtaacaacgctcaccaagtctgccaaactacggcctaacctatccagcctccaaacgaccaa
cgtatccccaggtctaagagcctttaagcagttatcaagttcgggtctagcggcattcttcccactggccttgtcctcgtaaatgatctc
gcaaccagcagcagccaacgcatctctctgcaaatgcaggttctgatcgtcggttgaaaccctagcgtagccaattttttgattcatg
tacgtttttttaacatagaattttatacaagtattttaacatgtatatagattaaattccaatcaacttgtgtcgttttcagaagacggctgc
actgaacgtcagaagccgactgcactatagcagcggag

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. What antibiotics were these organisms resistant to?
2. What mechanisms (structures) were proposed for the transfer of resistance genes?




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