Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 20

• Culture #20 - Set A

aaattcttctttttatgcaaccgcttgctctatactatttttgtagacgtttacaaaatgttgtcgaattagactatgcttttggggaggatgatcg
gtttgaccttgtatcgaagtctatggaaaaaagggtgtatgcttctactaagccttgtgctgtctctgaccgccttcattggctcaccctctaat
acggcgagcgccgcggttgcagatgattttcaggcttccgttatgggaccactggcgaaaatcaacgactggggttcctttaaaaagcaa
ctgcaaacgttgaaaagcaatggcgtgtatgccatcactaccgacgtctggtggggctatgtggagagcgcaggagataaccagttcgat
tggagctactacaaaacctatgcagatgccgtgaaagaagcgggactgaagtgggttcctattatctctacgcataagtgtggaggaaat
gtgggagatgattgcaacatcccactgccaagctggctgtcaagcaagggtagtgcagatgaaatgcagttcaaggacgaaagcggct
atgccaataacgaagccctttccccattgtggagcgggactggcaaacaatatgacgagttgtatgcctcattcgctcaaaactttgccggg
tataaaagtatcattcccaaaatttatttaagcggcggtccttccggggaattgcgctatccgtcctactacccggcagcgggctggagttacc
cgggacgaggcaagttccaagcctatactgaaacagccaaaaagtcattccgcacagctatgaatgacaagtacggatcattggacaa
gattaatgctgcttgg

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?
List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. The gene coding for beta amylase was cloned in what type of bacteria?
2. Does the sequence shown contain a translational stop codon? If not; why not?
3. What type of sequence occurs immediately upstream of the signal sequence indicated by italics (coding for 38 amino acids)?



• Culture #20 - Set B

gaggccgatagtctggaaaaactcgaagccctgctaccctggaacgtcccgctggagcccattgcaaaaaaagtggcgcaat
acaacgaaggcaagtaggtcgatttagcggcgcttacgggagaacagcttgccgggcttgcatcggccatgcgctaatcctgtg
gcataaccataaggggataaacaatgtggcagtggcttggaaaagtgcggtggttgctggtggtgatagcgctgctgggtggca
aggtcatggcggcgtctgaactggatagcgcgctggccagactcgagcagcagcatcatgggcgtctggggctggcctatatcg
atagtggcagcggcgagagttacagctatcgcggtgaggagcgttttgccttttgcagcaccttcaaggcggtgctggcgggggc
ggtgctgcagcgaagcgtcagtcagcccggtttgctcgataagcgggtgcattatgcggccacggatctgctcgcctatgccccca
tcaccaagacgcatctggataaagggatgcgcatcggtgaactggccgcagcggcggtgcaatatagtgacaacacggcggc
caacctgctgctgcaagaaattggcggcgtccaggcgctgaaccggtttgtgcaggggttgggagatccggcattccgtctggat
aggatagagcctcaccttaatagtgccgagccaggagatgtgcgcgataccaccacgccgctggccatggcccacaccctgcag
gccatgactctgggcaaggggtta

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. Where were ESBLs first characterized for members of the family Enterobacteriaceae?
2. What are ESBLs and what do these enzymes do?





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