Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 2

• Culture #2 - Set A

atgaaaaaaacagcatttacattacttttattcattgccctaacgttgacaacaagtccacttgtaaatggtagcgagaaaagcgaagaaataaatg
aaaaagatttgcgaaaaaagtctgaattgcagggaacagctttaggcaatcttaaacaaatctattattacaatgaaaaagctaaaactgaaaa
taaagagagtcacgatcaatttttacagcatactatattgtttaaaggcttttttacagatcattcgtggtataacgatttattagtagattttgattcaaa
ggatattgttgataaatataaagggaaaaaagtagacttgtatggtgcttattatggttatcaatgtgcgggtggtacaccaaacaaaacagcttgtat
gtatggtggtgtaacgttacatgataataatcgattgaccgaagagaaaaaagtgccgatcaatttatggctagacggtaaacaaaatacagtacct
ttggaaacggttaaaacgaataagaaaaatgtaactgttcaggagttggatcttcaagcaagacgttatttacaggaaaaatataatttatataactctg
atgtttttgatgggaaggttcagaggggattaatcgtgtttcatacttctacagaaccttcggttaattacgatttatttggtgctcaaggacagtattcaaat
acactattaagaatatatagagataataaaacgattaactctgaaaacatgcatattgatatatatttatatacaagttaa

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
Note - Students must access the full-text article from Lancet to answer the questions.
1. Describe the BLA Operon (name the genes present, what they code for, and what functions are associated with the proteins coded for).
2. How do leukocidins kill phagocytic WBCs?
3. What are superantigens?



• Culture #2 - Set B

gcgaaaaattaattgttatctctttttttatctacttataaattgacctttttcctaaaaagcaaagcccaaaattggcgatgatatatggcg
ttattatcgccatcgacttgaataattatttattaaaataataagtagagctagtgtatttattatcagggaggttcagatatagctaattatggat
aataagcgaacacagcgggatattatatttagcataatatggattatttggtcatgtgcattaatggctttttggcgatatcaaatagctggtga
gcttttgatcacccttatcctaatttttagcattattatttactctataactttgaggttaaaaaaaacggcaatgtttagtcgaaaaacagaaac
accaaaagccgcggagcctattgctccagttatggcagaagagaagaagcctatgccggagcaaaaattgtataccattattgctaaagg
cactgtatttcaaggtgatattaacgttgatggtgatattcaaatttggggtaaaatttcagggaatatcaatgtaaaagatggcgtgatccgt
gttatgcatgcaggccaagttgaaggggaattgacggcgccagacatcattattgatggttttgttaaaggtatttgtgccgcaaacaatcttg
atattctagagcatggtgaactacgtggcactagtcgttgtggcagtatgtcaattaaacgtggcggtgtctttactggacaatctgaacaggtt
gaacaccaagctaaagccgttgcaca

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. How is swarming characterized in these bacteria?
2. What is the function of the flagellar master operon flhDC?




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