Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 18

• Culture #18 - Set A

ctatttggggtgagtagataattgagccgcttttcaaactttttaatggtgaaaaaataatccatctcactcttgatgaagtaagacggatt
attgttgtttaaattagtactgtcttttaaagtgctttaaaaagagattgagcaggtatagtatttccttagtatttgttattctatgaaaatctct
agtttaaatcaatttatgtatgttattttcctattcaggtttatggttcaaatcattatctctaaaaaactagacattggagtaaaaggtatcat
ttatctgaatattgtaattatgatctcaaaaccgtagaaaatagtgaagatatgatttacctattttgtactgtttcttaatgttaattttgttccta
gtttagtaagtgttggtatttttcgaggtgctaataattgtttattgagaatatccatagctgttcgtcccatttcttctgtatagacagtgacact
agaaagtggaggatacacttgtttagctagagttgtatcgttaaaagagataatttgaatgtcgtcagggaccttgataccattttcctgaa
gtgctctgagtgcaccaatagctagactatcactagccgcaaagtaagcatcaggtaaagtagctccactcttaatcttagaatcaagaa
gttcatagccagattggacagtgaagtcaccagtcagaataaaaagagggtcatagattcccttttccatacagtagttccgataagaac
gtaaacgaggatcagagatgatttcagttgcatctgttgttttttcttgcccaatgagtaggcctatattattacaaccttgttctttaagataac
agagtgccgattgtacggagttttcaaaatcagtagtaacacatggatgtcct

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?
List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. When was the lactose operon of E. coli discovered?
2. Where is the b-galactosidase gene of this bacteria located relative to the permease gene?
3. Cultures of this bacteria do not usually ferment galactose. Is this because they lack genes coding for enzymes that would allow galactose utilization to occur, or is it because these genes are not being induced?



• Culture #18 - Set B

ccaggatcctggcgagacactataagcaggcgctggaaggacaaatatcgaattgatgatggaattccatcatcaataaattgcttgtca
aaatagcgggagtcgcagataatccgctgcgacttatcactctcaaggaatcagaaatgataaaaagttcgtggcgtaaaattgcaatgc
tagccgccgttccgctgctgctggcgagcggcgcactgtgggccagtaccgatgctatccatcagaagctgacagatctcgagaagcgtt
caggcggcaggttgggcgtggcgctaatcaacacggcagataattctcaaatcttatatcgcggcgacgagcgttttgccatgtgcagcac
cagtaaagtgatggccgccgccgcggtattaaaacagagcgaaagcaataaagaggtggtaaataaaaggctggagattaacgcagc
cgatttggtggtctggagtccgattaccgaaaaacatctccagagcggaatgacgctggctgagctaagcgcggcgacgctgcaatatag
cgacaatacggcgatgaatctgatcatcggctaccttggcgggccggaaaaagtcaccgccttcgcccgcagtatcggcgatgccacctttc
gtctcgatcgtacggagcccacgctgaataccgccatcccgggcgatgagcgtgataccagcacgccgctggcgatggctgaaagcctac

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. Since the early 1980s these bacteria have been recognized as what?
2. In wild type strains of these bacteria, chromosomal b-lactamases are constitutively produced at low levels and affect the organisms susceptibility to ___________________ while overproduction confers resistance to what?



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