Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 15

• Culture #15 - Set A

tggagatacggctctttctgcaaatgaagcgagaatgaaagagaccttgcaaaaggctggattatttgcaaaatctatgaatgcctattcttat
atgttaattaagaatcctgatgtaaattttgaaggaattactattaatggatatgtagatttacctggtagaatcgtacaagatcaaaagaatgc
aagagcacatgctgttacttgggacacgaaagtaaaaaaacagcttttagatacattgactggtattgttgaatatgataccacgttcgacaat
tattatgaaacattggttgaggcgattaatacaggggatggagaaactttaaaagaaggaattacagatttacgaggtgaaattcaacaaaa
ccaaaagtacgcacaacaattaatagaagaattaactaaattaagagactctattggacaagatgttagagcatttggaggcaataaagatc
ttttgcaatcgattctaaaaaaccaaggtgcagatgttgatgcagatcaaaagcgtctagaagaagtattaggatcagtaaactattataaac

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. What four species belong within this group of bacteria?
2. Which newly described species is psychroduric?
3. What symptoms are associated with the emetic toxin and enterotoxins produced by these bacteria?



• Culture #15 - Set B

ttgtgaatcagcaaaacgccgggttattcttatttgtcgcttctttactcgcctttatcggccctcactcaaggatgtattgtggttatgcgttatttt
cgcctgtgtattatctccctgttagccaccctgccgctggcggtacacgccagcccgcagccgcttgagcaaattaaactaagcgaaagcca
gctgtcgggcagcgtaggcatgatagaaatggatctggccagcggccgcacgctgaccgcctggcgcgccgatgaacgctttcccatgatg
agcacctttaaagtagtgctctgcggcgcagtgctggcgcgggtggatgccggtgacgaacagctggagcgaaagatccactatcgccag
caggatctggtggactactcgccggtcagcgaaaaacaccttgccgacggcatgacggtcggcgaactctgtgccgccgccattaccatga
gcgataacagcgccgccaatctgctgctggccaccgtcggcggccccgcaggattgactgcctttttgcgccagatcggcgacaacgtcacc
cgccttgaccgctgggaaacggaactgaatgaggcgcttcccggcgacgcccgcgacaccactaccccggccagcatggccgcgaccctg
cgcaagctgctgaccagccagcgtctgagcgcccgttcgcaacggcagctgctgcagtggatggtggacgatcgggtcgccggaccgttga
tccgctccgtgctgccggcgggctggtttatcgccgataagaccggagctgccaaacggggtgcgcgcgggattgtcgccctgcttggcccga
ataacaaagcagagcggattgtggtgatttatctgcgggatacgccggcgagcatggccgagcgaaatcag

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. What are ESBLs and what is their significance?
2. The bacteria that carry genes coding for ESBLs are frequently associated with what types of infections?




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