Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 12

• Culture #12 - Set A

ggaagcatcagcttagcaatcccgcataccctgtctggcacctaccagatcttgtagttgagccggtacgagcgttctgtgttttatg
caatccacatcagcgaccagggatgctggctatttgaaacacttcacggaatgacgctgaaagtcttcgcgacctcgtctgtcgca
ccttaacgaaagcattgcgaatccattaccgacaggtttccaaaagaaacccgggatgaaactcctattgcatttcgaaaattgga
aacgacaggcgaacatatgtaacgcgaaatttcaccctacgtataaacaatgcgcccagcgaatatcgctcccttaccgagcga
cgaactcctgcgcgccagcgaataaccggtgccgcgagggaaaagtttctccggcatacctggagagccctctcggaggcggcg
catgaacggtcagcggtacagggaaacacccctcgacatcgagcgtctgcggcgcctgaatcgcgccacggtggagcgctacat
ggcaatgaagggggccgaacggttacagcggcacagcctgttcgtcgaggacggctgcgccggcaactggaccacggaa
agcggcgaa

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Include abstract from NATURE (Medical significance of the organism being investigated)

Find the article that provided the reference sequence and answer the following questions.
1. What is pyocyanin?
2. What are the pathophysiological effects of pyocyanin in human airways?
3. What effect do the phenazines produced by these organisms have on alveolar macrophages?



• Culture #12 - Set B

ccgcgaacgcgtgcgtcagattcaggttgaaggtctgcgccgtctgcgggaaatcctgcagggtcaggggcttaacatcgaagcgctgttccgt
gagtaagtgaacattcgtcaaaaaggccagtctgcgactggcctttttcttttgtaccgccagatgaataaccacaccaggcgctggcagccgac
aaaggccgcgtctctagcccatcattttacgcaccagtttggtaaacagcgcgcgctcctcatcgctcaacctgccgagaaattcctcatcaacgg
cgttaccgacaggttttcgggcattcaacaacgcttctccctcaggggtgagaaagacaaatcgccgacgtttgtccgccggatcgtgttcccgctt
aaccagattacggctctccattcgactgagcatctctgccagcgtggcttttgtgctgacggccacttcggtgagttcgacctgttcaatccctggatg
ctcggcaatcgagcgcatgaccgcgtactggggtttggtcaattccggcaattcgtgctgccagcgtgccgtatgctgttggaaaagctggcgtagc
aggtgaaatgcttcgtttcttaactccatgaaaaccccatcttaaaatacgtgttctttatgatagccgctcctggcggcttttataatccttcatttttaa
gctttaagtgcgatcgtgtacgaacaatattgccactgggtgcgttcaggcgtagttttatgacaaacgttcgtacacgaacaaatattgaggcgta
aatgagattgattgttggaatgaccggcgccaccggtgcgccgctcggcgttgcgcttttgca

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?
List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. The nucleotide sequence located downstream from the rpoS gene in Enterobacter cloacae and Kluyvera cryocrescens contains what genes?
2. What is the size of the insert sequence described in this article?
3. This article suggests that the insert being studied may be related to pathogenesis and what else?




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