Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 11

• Culture #11 - Set A

tggagatacggctctttctgcaaatgaagcgagaatgaaagagaccttgcaaaaggctggattatttgcaaaatctatgaatgcctattcttata
tgttaattaagaatcctgatgtaaattttgaaggaattactattaatggatatgtagatttacctggtagaatcgtacaagatcaaaagaatgca
agagcacatgctgttacttgggacacgaaagtaaaaaaacagcttttagatacattgactggtattgttgaatatgataccacgttcgacaatt
attatgaaacattggttgaggcgattaatacaggggatggagaaactttaaaagaaggaattacagatttacgaggtgaaattcaacaaaac
caaaagtacgcacaacaattaatagaagaattaactaaattaagagactctattggacaagatgttagagcatttggaggcaataaagatctt
ttgcaatcgattctaaaaaaccaaggtgcagatgttgatgcagatcaaaagcgtctagaagaagtattaggatcagtaaactattataaac

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. What four species belong within this group of bacteria?
2. Which newly described species is psychroduric?
3. What symptoms are associated with the emetic toxin and enterotoxins produced by these bacteria?



• Culture #11 - Set B

gcggagtacatcgcgttctgcacgcgctccggctgcgtctgcagcgagaccaattggctgttgtaggtcgcaagatcgttctgcgcgc
tttgcagatcgtcgagcgcctgagacaccctggattcaagctgtcgcaacgataacgtgctcagggttttacgggtgacctcatcatca
ggaacgtcgctcagcgcattcaggctgtctaccgcctgacgcattttcgccggcgcctgttcaacctgctgacgcagttgcacagtttc
agactttatacgctcaatcttatcgagcgtttctaaagtctgcgtcaggtcctgctgaactaatttttcctgcggcgtgagttctttttgctta
tttagcgtagtgagctggctttgaatgtcagaacggtcaggtaaatctgcagcctgaacctggctgaagctcagagtgccaactata
aggaccagtgcaaagacaatggtggaaatagcatgaggccaacgatttgtatgctgcatagtattaacatgaatggttgcaaatgc
cggagaataccgggggtcgtcgcagcgcgcagaatagcacttctccctgcgcgcgaatagcggcagagataagtaaaatttaccttta
tttacgcctgcggattcaggccatgacctcagacgaacttcgtagcgacgggcaggtctgatacatctcaatcagcgcatcgac
ataagcacgggcttc

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. For the last decade, these commensal Gram-negative bacteria have been rapidly emerging as what?
2. The pumps used by these bacteria to remove disinfectants and antibiotics from their cytoplasm (cytosol) use what as a source of energy?




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