Sierra College Department of Biological Sciences


Nucleotide Sequencing Results - CULTURE # 10

• Culture #10 - Set A

atgaaaaaatttatacttcctatattcagcatttctattctagtttctctcagtgcatgttcatctattaaaactaaatctgaagataattttcatatttctt
ctcagcaacatgaaaaagctattaaaagctattttgatgaagctcaaacacagggtgtaattattattaaagagggtaaaaatcttagcacctat
ggtaatgctcttgcacgagcaaataaagaatatgtccctgcatcaacatttaagatgctaaatgctttaatcgggctagaaaatcataaagcaac
aacaaatgagattttcaaatgggatggtaaaaaaagaacttatcctatgtgggagaaagatatgactttaggtgaggcaatggcattgtcagca
gttccagtatatcaagagcttgcaagacggactggcctagagctaatgcagaaagaagtaaagcgggttaattttggaaatacaaatattggaa
cacaggtcgataatttttggttagttggcccccttaaaattacaccagtacaagaagttaattttgccgatgaccttgcacataaccgattaccttttaa
attagaaactcaagaagaagttaaaaaaatgcttctaattaaagaagtaaatggtagtaagatttatgcaaaaagtggatggggaatgggtgtt
actccacaggtaggttggttgactggttgggtggagcaagctaatggaaaaaaaatccccttttcgctcaacttagaaatgaaagaaggaatgt
ctggttctattcgtaatgaaattacttataagtcgctagaaaatcttggaatcatttaa

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?

List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. What types of diseases are caused by these bacteria?
2. What antimicrobial agents have recently become the drugs of choice for controlling these bacteria, and what has occurred to present a problem with this practice?

3. The bacteria that served as the subject of this research paper came from what source?



• Culture #10 - Set B

gtcgacttcatcctgataaaacacaggatgtgcagtctgctcctgagccagcgcctgatcaatgacaagccgtttttcttcatagtgcggatctttga
ttttcagcgttggcgctgccctgcgccagaccatatctgcctgcctcagatatcggtgcagggtggagcggtgcagcgtcacatcaaaaagtcgg
ttgatgacgagtgccagaagctccgtgctccagcgtgagcgcagccagccaaaatccttcggggaacgctgtaccaatagtggcaagagctga
aggatatcagcgaccggccagcgtggagcacgtccgggtctgaggctttttagtccttcaacaccatgtaaagtaaaccagtttatccatcgacca
acggatgaacgggcggcacagagtaatctggcaacgtcagtgacggtcattccctgatgcagcatcagcatggcgataagccgtcgcgcatggt
ttttatcgtgggtttgttgggcctctttgcgcatcagttgtcgttcttcatcaggaattgctgctatgatcggcatcgctcagtccggttggtgatttgtgatg
tttggcgattgatcagatcgcacaatccgggctgagttccctttcagtgatctactattttgcgaagctatttagtgcacactaatcgatttttcagacaa
cctttctcgcctggcgtgagttttcgttcgactgaacctataaaaaggctctgcattctgtcctgtagtgcgccaggcctaataacgcctcccgtttaattc
cggcccggtcgtacattggttctgttcatttatgggatcgcgactttttcgttgtaa

Questions for students include:

What is the name of the organism type from which this nucleotide sequence was taken?
What feature of the organism type is represented, i.e., what does this sequence code for?
List taxonomic information, i.e., lineage for the organisms (cut and paste from NCBI).

Find the article that provided the reference sequence, and answer the following questions.
1. What size is the virulence plasmid in S. enterica serovar Typhimurium?
2. What structures do genes on the pef operon code for?
3. The complete DNA sequence of this plasmid could provide what?



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